This study aims to investigate the bearing capacity of the high-plasticity clay soil with bacteria stabilization (Bacillus subtilis). The growth of bacteria with the phase of 6 days was used as a stabilizing agent. The assessment of bearing capacity was based on unconfined compressive strength value, California Bearing Ratio (CBR), and modulus of soil reaction as a subbase layer. The test results show that the compressive strength value tends to increase with the addition of 2% to 6% bacteria and decrease with 8% bacteria. This phenomenon corresponds to the increase of CaO value due to Microbial-Induced Calcite Precipitation (MICP) microstructurally. The compressive strength value curve pattern increases proportionally with curing time of 3, 7, 14, and 28 days. According to the California Bearing Ratio (CBR) value range criteria (20% ≤CBR˂40%) for subbase layer, the use of 3.5 to 6% bacteria can technically be functionally justified as a road material with the modulus of stabilized clay soil reaction value of 68 to 110 kN/m 2 /mm. The results of this study have technical implications for the significance of the use of Bacillus subtilis bacteria as the biotechnology stabilization material to increase the bearing capacity and modulus of soil reaction with high plasticity clay.

Abstrak: Kegiatan PKM ini diselenggarakan di Desa Wawoangi Kecamatan Sampolawa Kabupaten Buton Selatan. Masalah yang dihadapi mitra adalah Kurangnya wawasan dan kesadaran masyarakat lokal akan pentingnya pembangunan di bidang kepariwisataan. Tujuan dari PKM ini dilaksanakan ialah untuk memberikan pendampingan pembinaan pengelolaan pariwisata dan arahan pengembangan pariwisata. Program pengabdian masyarakat ini menyimpulkan bahwa peran modal sosial dapat digunakan untuk masyarakat dalam bentuk pengetahuan lokal, yang kemudian dapat dimanfaatkan sebagai sarana pendampingan, pembinaan, pengelolaan pariwisata dan arahan pengembangan objek wiata kepada pemerintah desa dan masayarakat Desa Wawoangi. Dengan dikelolanya objek wisata di Desa Wawoangi ini akan mendorong laju perekonomian rakyat, karena dengan adanya tujuan wisata di Desa ini akan mampu mempromosikan semua sektor sektor ekonomi yang ada di desa, baik itu dari kerajinan, pertaanian, dan budaya.Kata kunci: Pariwisata, Ekonomi Desa

The goal of this research is to synthesize and characterize Fe3O4@Chitosan-AgNP nanocomposites in order to determine their antibacterial activity. The research methods include the synthesis of Fe3O4@Chitosan-AgNP nanocomposites, as well as the characterization of nanoparticles using scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), Fourier transform infrared (FTIR) analysis, and subsequent antibacterial activity tests. The study’s findings demonstrated the successful synthesis of Fe3O4@Chitosan-AgNP nanocomposites, followed by nanoparticle characterization using SEM, TEM, XRD, and FTIR. Based on the XRD results, the conjugation of Fe3O4@Chitosan-AgNP nanocomposites has been successfully formed, as evidenced by the appearance of characteristic peaks of Fe3O4, chitosan, and AgNPs. According to the FTIR results, the interaction between chitosan-AgNPs and conjugated Fe3O4 occurred via the N atom in the NH2 group and the O atom in the OH group, and C=O. The SEM and TEM images also show that the Fe3O4@Chitosan-AgNP conjugation is a nanoparticle-based composite material. The combination of nanocomposites Fe3O4@Chitosan-AgNPs has antibacterial activity, inhibiting the growth of bacteria such as Bacillus cereus and Escherichia coli.

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