Abstract-The purpose of this study was to ultrasonically characterize infarcted human myocardial tissue at the microscopic level by scanning acoustic microscopy. Infarcted myocardial specimens from ten cases with acute myocardial infarction were studied. Specimens were formalin fixed, para& embedded and sectioned to lo-pm thickness. A specially developed scanning acoustic microscope system, operating in the lOO-to 200-MHz ultrasound frequency range, was used for the measurements.

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It was verified that the frequency dependence of magnitude and phase of the reflection coefficient gave characteristic patterns against the specimen thickness, so that specimen thickness could be determined by analysis of those characteristic patterns. As a result, attenuation constant and sound speed in thin tissue specimens were determined with a non-contact procedure using a scanning acoustic microscope.

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