In this work, Yb 3+ -immobilized hydrophilic phytic-acid-coated magnetic nanocomposites were prepared through a facile route and used to selectively separatrf bovine hemoglobin. Hydrophilic phytic acid (PA) was coated onto the magnetic Fe 3 O 4 -PEI via electrostatic interactions, followed by finally chelating with Yb 3+ ions, which could produce specific protein binding sites at room temperature in water, and complex instrumentation was not necessary. The performance of as-prepared hybrids (Fe 3 O 4 -PEI-PA-Yb 3+ ) was assessed by selectively isolating bovine hemoglobin (BHb). The obtained maximum binding capacity was 347.3 mg g −1 . The retained BHb could be eluted under simple elution via using 0.1 M of Na 2 CO 3 , giving a recovery of 83%. Moreover, the generation of nanocomposites was demonstrated. In addition, the PA and PEI could improve the hydrophilicity of nanoparticles and further reduce the nonspecific adsorption. Therefore, such nanocomposites were successfully employed to selectively bind and separate BHb from bovine serum as verified by SDS-PAGE and MALDI-TOF MS analysis, providing a new perspective for the isolation of heme proteins in proteomics.

No abstract

It is highly required to develop well-designed separation materials for the specific isolation of certain proteins in proteomic research. Herein, the new type of metal–organic framework (MOF)-derived polymer-mediated magnetic hollow nanocages was fabricated via stress-induced orientation contraction, which was further applied for specific enrichment of proteins. The core–shell nanocomposites comprised of polymer-mediated ZIF-67 cores and polydopamine (PDA) shells, after annealing, generated magnetic hollow carbon nanocages with hierarchical pores and structures. Particularly, the magnetic carbonized PDA@F127/ZIF-67 hollow nanocages exhibited a remarkable adsorption capacity toward bovine hemoglobin (BHB) up to 834.3 mg g–1, which was significantly greater than that of the directed carbonized ZIF-67 nanoparticles. The results also exhibited the notable specificity of the obtained nanocages on complex biosamples, including intact mixed proteins and fetal calf serum. The hierarchically hollow porous structure greatly improves the specific surface area and reduces the mass transfer resistance, leading to enhanced high adsorption for target protein BHB. This novel method will be promising for the applications in purification and enrichment of biomacromolecules for complex biosamples, which successfully solve the problem of low adsorption efficiency and tedious separating process of the previous MOF-derived materials.

No abstract

No abstract