We studied the effects of antibodies to glutamate and GABA administered intranasally 1 h before and immediately after stress on the development of stress response in C57Bl/6 mice. Antibodies to glutamate administered 1 h before stress prevented the development of the stress-reaction judging from behavioral parameters in the open field test and hyperalgesia test, while antibodies to GABA potentiated the severity of stress-induced behavioral disorders: total behavioral activity in the open field decreased significantly. When administered after stress, antibodies to glutamate did not reduce, but exacerbated stress reaction; antibodies to GABA also exhibited a stress-potentiating effect.

We studied the dose-dependent effect of antibodies to glutamate on the stress response in C57Bl/6 mice. The antibodies were administered immediately after stress exposure. Intranasal administration of antibodies to glutamate in doses of 150 and 250 μg/kg immediately after stress exposure was shown to reduce the stress response under conditions of combined restraint stress. This effect was most pronounced after treatment with antibodies in a dose of 250 μg/kg: we revealed a decrease in the number and severity of erosive and ulcerative lesions in the gastric mucosa, i.e. anti-glutamate antibodies have a protective effect.

The production and role of autoantibodies against neurotransmitters glutamate, gamma-aminobutyric acid, and norepinephrine were studied in rats with experimental neuropathic pain syndrome induced by sciatic nerve transection. Nerve transection in rats was followed by behavioral reaction of autotomy (self-mutilation of the operated limb), which often accompanies neuropathic pain syndrome. The development of neuropathic pain syndrome was accompanied by increased production of autoantibodies against glutamate, gamma-aminobutyric acid, and norepinephrine. A negative correlation was found between the amount of autoantibodies against neurotransmitters and severity of neuropathic pain syndrome. Our results suggest that antibodies against glutamate and norepinephrine exhibit protective activity.

We studied the effect of antibodies to glutamate and GABA (active immunization with conjugates of glutamate--bovine serum albumin and GABA--bovine serum albumin) on the course of combined water-immersion stress in C57Bl/6 mice. Preimmunization of animals with the conjugate of glutamate--bovine serum albumin was accompanied by strong production of antibodies to glutamate, which reduced the majority of signs of the stress response. Antibodies to GABA had no effect on the development of stress.