Abstract.The new approach to enhancing the selectivity of sorption separation of metal ions was suggested. The approach is based on using mixed sorbents, which may be obtained, e.g. by simultaneous immobilization of complexing reagents on silica gel and reverse-phase silica gel (C16, Ph). The methods are developed for the simultaneous determination of two (Co-Fe, Co-Zn, A1-Mn) or three (A1, Mn and Fe) elements in the concentration range 2.10 -6-7.10-4M with the performance of 21-36 samples per hour.Key words: mixed sorbents, continuous flow analysis, simultaneous determinations, reverse-phase silica gel.The combination of sorption separation with simple detection technique, e.g. photometric, requires selective sorbents. We are developing a novel approach allowing the preparation of the sorbents with an increased selectivity -the simultaneous immobilization of different complexing groups (reagents). Varying relative content of the groups under constant total capacity of the sorbent makes possible to change the retention of ions [1]. The authors of [2] have used HPLC sorbents dynamically coated with mixtures of crown-ethers; however, to get maximal effect it is desirable of "mix" functional groups with different donor sets. Related theory and application to thin-layer chromatography were considered in our previous paper [1]. To our knowledge, no applications of the sorbents with different complexing groups were reported for sorption concentration; all the published data are related to chromatographic methods.The goal of the work is the further development of the approach. We describe the methods of preparation of mixed sorbents, the properties of a number of such sorbents, and their application in continuous flow analysis (CFA).

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Monocyte migration from the peripheral blood to the uterine decidual tissue is essential for the regulation of placental development. We evaluated the phenotypical changes in the peripheral blood monocytes in pregnant women. The peripheral blood counts of monocytes expressing CD11b, CD47, and integrin β7 were elevated in women with normal gestation in comparison with nonpregnant women; the intensity of CD62P, CD11b, CD11c, CD29, CD31, and CD54 expression was higher in pregnancy. The counts of monocytes expressing adhesion molecules were similar in normal pregnancy and gestosis. Gestosis was characterized by higher counts of monocytes expressing IFN-γ receptor (CD119) and more intense expression of this receptor. Changes in the monocyte phenotype can promote their adhesion to the uterine vascular endothelium and further migration of these cells to the decidual tissue.

Introduction. Soy pulp, or okara, is a soy milk by-product. It contains a complex of valuable macro- and micronutrients, including fat-soluble vitamins. Recently, there has been a growing interest in replacing synthetic antioxidants with a mix of natural tocopherols. Soy beans and their by-products contain vitamin E and therefore can be used in food production, cosmetic, etc. Study objects and methods. The present research featured soy pulp. The content of tocopherols in the soy product was determined by high performance liquid chromatography using Sigma Aldrich standards and corresponding calibration dependencies. The research also involved spectrophotometry for determining tocopherols in standard solutions followed by chromatographic separation using fluorimetric detection. Results and discussion. To determine tocopherols in the okara, the test samples were treated with hexane. The filtered solution was exposed to low temperatures, after which the phases formed by centrifugation were separated. The samples were tested for all forms of tocopherols with the exception of β- and γ-forms. The chromatographic separation of the peaks of these forms was difficult due to the fact that their structural formulas were similar and differed only in the arrangement of methyl groups, i.e. the ortho- and paraposition. As a result, it was decided to determine the sum of the β- and γ-forms, which did not affect the objectivity of the analysis, since, according to published data, the content of β-tocopherol in soy products stays within 5%. Conclusion. The proposed method for sample preparation made it possible to improve the separation of the lipid fraction, which had a positive effect on the results of the analysis. The method also determined not only the quantitative, but also the qualitative content of tocopherols in the product. This reduced the risk of malfunctioning chromatographic equipment: the method proved more economical in terms of labor and reagent use. The obtained results corresponded to the existing scientific data on the content of tocopherols in soy products. The content of tocopherols in soy okara was 109 mg%.

We examined the structure of psychoemotional sphere of 153 pregnant patients in different terms of pregnancy and 48 non-pregnant women. Our data demonstrated that first-term pregnancy is accompanied by a significant emotional stress. In second-term pregnancy those changes are more positive, at third-term pregnancy the level of the patients feels is significantly increased and the psychoemotional sphere is stabilized. Thereby the specific features of nervous system are influence the status of the pregnant women. Evidently pregnant patients with the high level of anxiety, introversion, psychotic and nevrotic characteristics are in need of the increased attention of the obstetric-gynecologic specialists, anaesthesiologists and psychologists to make correction of the psychoemotional sphere when preventive preparation of birth and the choice of anesthesia delivery.