Acute renal injury is characterized by decrement of glomerular filtration rate (GFR), resulting in nitrogen metabolic end product retention such as blood ureum. Aminoglycoside nephrotoxic injuries are common in elderly, with mortality rate 50-70%. Tempuyung has been empirically used in relieving renal problems as it contains several active antioxidants. This study aimed to analyze the protective effect of ethanol extract tempuyung leaf (EET) against gentamicin induced renal injury viewed from blood ureum level. The method used was true experimental laboratory, 16 wistar male rats, divided into 4 groups (n=4): negative control group (CMC), positive control group (Gentamicin 80 mg/kgBW), 1 st treatment group (EET 100 mg/kgBW + Gentamicin), 2 nd treatment group (EET 200 mg/kgBW + Gentamicin) for ten days. On the 11 th day, blood serum were taken and the ureum level were analyzed. Data was analyzed by ANOVA and post hoc LSD test. Result showed positive control group had the highest blood ureum level (29.50 1.29 mg/dL) compared to negative control group (14.25 1.26 mg/dL), 1 st treatment group (26.50 0.58 mg/dL), and 2 nd treatment group (22.25 1.26 mg/dL) with significance difference (p<0,001). In conclusion, the result suggested the protective effect of EET against gentamicin induced renal injury. AbstrakGangguan ginjal akut ditandai dengan penurunan laju filtrasi glomerulus (LFG), menyebabkan retensi sisa metabolisme nitrogen, satu diantaranya adalah ureum darah. Insidensi nefrotoksisitas akibat obat golongan aminoglikosida umum terjadi pada kelompok usia lanjut dengan angka mortalitas 50-70%. Tempuyung telah dipakai sejak dulu sebagai obat tradisional, untuk mengatasi gangguan ginjal karena kandungan sejumlah antioksidan. Tujuan penelitian ini untuk melihat efek proteksi Ekstrak Etanol Daun Tempuyung (EET) terhadap kerusakan ginjal akibat induksi gentamisin dilihat dari kadar ureum darah. Metode berupa eksperimental laboratorik, 16 tikus Wistar jantan dibagi menjadi 4 kelompok (n=4): kelompok kontrol negatif (CMC), kelompok kontrol positif (Gentamisin 80 mg/kgBB), kelompok perlakuan 1 (EET 100 mg/kgBB + gentamisin), dan kelompok perlakuan 2 (EET 200 mg/kgBB + gentamisin) selama sepuluh hari. Pada hari ke-11 darah diambil dan diperiksa kadar ureum.darah Data dianalisis dengan uji statistik ANOVA dan uji beda rata-rata LSD. Hasil menunjukkan kelompok kontrol positif dengan ureum tertinggi (29.50 1.29 mg/dL) bila dibandingkan dengan kelompok kontrol negatif (14.25 1.26 mg/dL), kelompok perlakuan 1 (26.50 0.58 mg/dL), dan kelompok perlakuan 2 (22.25 1.26 mg/dL), dengan perbedaan yang bermakna (p<0,001). Kesimpulan hasil menunjukkan efek proteksi EET terhadap kerusakan ginjal akibat induksi gentamisin.Kata kunci: gangguan ginjal akut, gentamisin, laju filtrasi glomerulus, tempuyung, ureum HasilHasil pengukuran kadar Ureum pada hewan coba yang telah mendapatkan perlakuan selama 10 hari menunjukkan hasil sebagai berikut:Gambar 1 Kadar Rerata Ureum (mg/dL) SD Gambar 1 menunjukkan hasil penelitian menggun...
Premature skin aging occurs due to the increased formation of reactive oxygen species (ROS), which causes oxidative stress, DNA damage, and collagen degradation. This study investigates the protective effect of Scenedesmus dimorphus polysaccharides (SDP) as an antioxidant and anti-aging agent on an aging rat model induced by D-galactose (D-gal). This study used 48 male Wistar rats divided into six groups: (1) normal control, (2) polysaccharide control, (3) aging control induced by D-gal 0.25 mg/g bw/days, (4) drug control (vitamin E) treated twice with SDP in an aging rat model, (5) D-gal + SDP (0.2 mg/g bw), and (6) D-gal + SDP (0.8 mg/g bw) treated with oral treatment and observed for periods of 2, 4, and 8 weeks. Giving SDP at a dose of 0.8 mg/g bw can increase superoxide dismutases and catalase activity and reduce malondialdehyde after 8 weeks of observation, which is better than giving vitamin E. The treatment of SDP can stimulate collagen synthesis and reduce advanced glycation end products. Histopathology shows an increase in the area of fibrocollagen proportions and deposition from the matrix on giving SDP, which is better than vitamin E, since SDP can repair skin tissue. Thus, SDP can be useful as an antioxidant and anti-aging agent in an aging Wistar rats model.
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