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0, 31,33, 40, 30.67, 46 dan 34% pada konsentrasi sampel berturut-turut 1,1x10 -10

Guaiacol, a phenolic compound is known as an anticancer. Dimerization of guaiacol has been done by biotransformation using peroxidase enzyme as biocatalyst. This enzyme was isolated from Indonesian plant, kailan (Brassica oleraceae var. alboglabra). Analysis of dimerization product was carried out by TLC, IR, LC-MS, and NMR. Whilst analysis of in-vitro cytotoxic activity was carried out by MTT method against breast cancer T47D and MCF7 cells. The result showed that the dimerization reaction gave O-para dehydroguaiacol. The in-vitro cytotoxic activity analysis showed that O-para dehydroguaiacol compound has potency as anti-breast cancer.

Abstract.Objective : In continuation of our antimalarial candidate drug discovery program on Indonesia medicinal plants especially from stem bark of Calophyllum bicolor. Metode : We extracted of bioactive crude extract with hexane, acetone and methanol from stem bark of Calophyllum bicolor and evaluated their antimalarial activity by using parasite Plasmodium falciparum in vitro. Results: Methanol fraction showed most active and potent antimalarial activity dose dependent in in vitro experiments with IC50 = 0.52 ppb, and hexane fraction showed moderate antimalarial activity, but dose not dependent, while acetone fraction have not antimalarial activity. TEM (Trasmission Electron Microscopy) analysis confirmed a remarkable reduction of hemozoin in the presence of the bioactive fraction. Conclusion: The results suggest that the antimalarial activity of Calophyllum bicolor is due, methanol fraction showed the best in vitro antimalarial activity then another fraction.

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