BACKGROUND: One of South Sulawesi’s huge brackish water fishery product is milkfish (Chanos chanos). Scales are wasted in milkfish processing. However, they are a good source of chitosan, which has been found to promote anti-inflammation, wound healing, and bone regeneration. AIM: This study aims to determine the effect of milkfish scales waste on the inflammatory response of wound healing after tooth extraction by tumor necrosis factor-alpha (TNF-α) and interleukin (IL)-6 analysis. METHODS: This is a post-test-only control group design study. Thirty-two Cavia cobaya were divided into four groups: (1) Socket preservation using milkfish scales chitosan, (2) milkfish scales chitosan + bovine xenograft, (3) bovine xenograft as a positive control, and (4) placebo as a negative control, then were sacrificed on 3rd, 7th, 14th, and 28th days. The mandible jaw specimen was taken for immunohistochemical analysis to determine the levels of TNF-α and IL-6. The data were analyzed using the Kolmogorov–Smirnov test, Levene’s test, and one-way analysis of variance. RESULTS: On days 3, 7, 14, and 28, groups with chitosan added showed lower levels of TNF-α and a faster decrease in IL-6 expressions compared to those without chitosan. CONCLUSION: Milkfish scale chitosan suppresses TNF-α and IL-6 production, thus reducing inflammation in socket preservation.

Background: Non-carious cervical lesions (NCCLs) are commonly associated with multiple gingival recessions. These cases cause aesthetic issues, dentin hypersensitivity and increased plaque accumulation. Therefore, they require multidisciplinary treatments. Zucchelli proposed the combined periodontal–restorative approach using envelope coronally advanced flap (CAF) in managing multiple gingival recessions with NCCLs. Objective: This case report highlights the effectiveness of Zucchelli’s modified envelope CAF in combination with a restorative approach for the treatment of multiple Miller class- III gingival recessions with type-3 NCCLs. Case Report: Zucchelli’s approach was used to determine maximum root coverage (MRC). Restorative treatments in covering the NCCLs were limited to 1 mm apical to the calculated MRC. Modified envelope CAF was performed with full and partial thickness flap. After root exposure, root planning was done and restorative finishing was performed to acquire the ideal restorative finish line without marginal overhanging. Root surfaces were conditioned for 2 min. Adjacent interdental papillae were deepithelialized, and the flap was repositioned and secured using sling sutures to establish a tight seal, allowing the clot between the root concavity and the flap to mature. Periodontal dressing was applied. Results: After 12 days, healing was uneventful with good aesthetic and functional results. Complete root coverage according to MRC predictions was obtained, and periodontal health was normal. Conclusion: Zucchelli’s modified envelope CAF with restorative approach shows good outcomes in treating multiple gingival recessions with NCCLs, given the correct determination of MRC. Keywords: gingival recession, periodontal plastic surgery, root coverage

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Background: One of the pertinent challenges the periodontists face today is the regeneration of periodontal tissue because of its complex structure. Periodontal tissue damage is characterized by bone and soft tissue loss. At present, one of the recommended materials for regeneration is PRF or platelet-rich fibrin. It is a regeneration material containing growth factors, prepared from the patient’s own blood, that can be used as a membrane. Objective: To see the efficacy of healing periodontal tissue using the addition of PRF. Method: A 31-year-old woman presented to the Department of Periodontology of Hasanuddin University Dental and Oral Hospital with the chief complaint of elongated teeth that often bled. Intraoral examination showed pocket depths of 6 mm and 5 mm in teeth 32 and 42, respectively. Radiographs showed radiolucent features in lower anterior teeth with horizontal bone loss. Chronic periodontitis of 32 and 42. Therapeutic intervention included initial therapy by scaling and root planning, followed by flap operation and regenerative therapy with PRF, membrane and bone graft. Results: One week post regenerative therapy with PRF, the clinical appearance seemed better and there was no complaint from the patient. Conclusion: The addition of PRF to periodontal surgery as a regenerative therapy can accelerate healing in the treatment of chronic periodontitis. Keywords: flap operation, platelet rich-fibrin, tissue regeneration