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BACKGROUND: One of South Sulawesi’s huge brackish water fishery product is milkfish (Chanos chanos). Scales are wasted in milkfish processing. However, they are a good source of chitosan, which has been found to promote anti-inflammation, wound healing, and bone regeneration. AIM: This study aims to determine the effect of milkfish scales waste on the inflammatory response of wound healing after tooth extraction by tumor necrosis factor-alpha (TNF-α) and interleukin (IL)-6 analysis. METHODS: This is a post-test-only control group design study. Thirty-two Cavia cobaya were divided into four groups: (1) Socket preservation using milkfish scales chitosan, (2) milkfish scales chitosan + bovine xenograft, (3) bovine xenograft as a positive control, and (4) placebo as a negative control, then were sacrificed on 3rd, 7th, 14th, and 28th days. The mandible jaw specimen was taken for immunohistochemical analysis to determine the levels of TNF-α and IL-6. The data were analyzed using the Kolmogorov–Smirnov test, Levene’s test, and one-way analysis of variance. RESULTS: On days 3, 7, 14, and 28, groups with chitosan added showed lower levels of TNF-α and a faster decrease in IL-6 expressions compared to those without chitosan. CONCLUSION: Milkfish scale chitosan suppresses TNF-α and IL-6 production, thus reducing inflammation in socket preservation.

BACKGROUND: Pinctada maxima pearl shell contains inorganic and organic materials that have a bone-like basic structure that facilitates bone remodeling. AIM: This study aimed to describe the effectiveness of Pinctada maxima pearl shells as bone graft material in the world of dentistry using an animal model. METHODS: Research uses Pinctada maxima pearl shell that was processed into hydroxyapatite Pinctada maxima (HPM) powder. Chemical and surface characteristics of HPM were performed with X-ray fluorescence (XRF), Fourier transform infra-red (FTIR), and X-ray diffraction (XRD), respectively. Thirty male guinea pigs were randomly assigned into three groups: Negative control (NC), positive control, and HPM. After 14–21 days of observation, guinea pigs were sacrificed. Bone formation was seen through immunohistochemical examination of osteoprotegerin (OPG) and bone morphogenetic protein (BMP2) expression. Data were analyzed through Shapiro wills and analysis of variance with a significance level of 5%. RESULTS: There was a high expression of OPG and BMP2 on days 14–21 in the HPM group when compared to the NC group with a significance level of 5%. CONCLUSION: HPM powder can be used as a promising bone graft material in the world of dentistry.

One of the basic requirements for succesfull transplantation is an adequate size of recipient site with sufficientalveolar support. If the transplantation is performed into a fresh extraction site, the recipient site can easily beadjusted to the transplantation. However, the situation is different in patient with premolar aplasia or early molarloss with marked atrophy of the alveolar process, need additional surgical procedures like bone autograft orsplitting osteotomy of the alveolar process. This paper discussed the effect of bone autograft and splitting osteotomyon root development of transplanted immature third molars. The conclusion is these additional surgical prosedureshad a negative effect on the pulpal and periodontal condition of transplantated immature third molars. This may berelated to revascularization disturbances of pulp due to nutrient insufficiency of the recipient site.