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This paper (1) describes a method for short-term, reproducible, quantitative measurement of the effects of experimental procedures on the migratory powers of reticuloendothelial cells in culture. Although splenic tissue was used in the preliminary work, the technique is adaptable for observations on mature and precursor motile cells in bone marrow and other organs.A 5-mm length of woven Fiberglas sleeving is prepared by repeated washing in xylol, alcohol, water, dilute HC1, NH4OH, and double-distilled water. Its lower 1.5 mm is made into a well-type receptacle by filling its interstices and the lower end with Diatex. (Diatex is a toluol solution of a transparent acrylic acid, plastic compound which hardens on drying and which can be autoclaved or sterilized in a heat oven. It has proved to be nontoxic in over 1200 tissue cultures. )The prepared sleeving is attached along its length to a piece of cover glass (Fig. 1). In the developmental stage of this project, half of a spleen from a 12day chick embryo, removed with the usual aseptic precautions, was used as test material. This piece of tissue is slipped into the open end of the Fiberglass sleeving, which with its attached cover glass is then placed in a Kahn tube containing 0.7 ml of supernatant, which consists of 0.25 percent human serum protein in Hanks' balanced salt solution. The Kahn tube is closed with a sterile serum-type rubber stopper containing a No. 20 hypodermic needle (fitted at its upper end with a cotton-plug bacterial filter).Any free, nonmigratory, dead or damaged cells from the fragment of tissue LO W o Z tq This paper (1) describes a method for short-term, reproducible, quantitative measurement of the effects of experimental procedures on the migratory powers of reticuloendothelial cells in culture. Although splenic tissue was used in the preliminary work, the technique is adaptable for observations on mature and precursor motile cells in bone marrow and other organs.A 5-mm length of woven Fiberglas sleeving is prepared by repeated washing in xylol, alcohol, water, dilute HC1, NH4OH, and double-distilled water. Its lower 1.5 mm is made into a well-type receptacle by filling its interstices and the lower end with Diatex. (Diatex is a toluol solution of a transparent acrylic acid, plastic compound which hardens on drying and which can be autoclaved or sterilized in a heat oven. It has proved to be nontoxic in over 1200 tissue cultures. )The prepared sleeving is attached along its length to a piece of cover glass (Fig. 1). In the developmental stage of this project, half of a spleen from a 12day chick embryo, removed with the usual aseptic precautions, was used as test material. This piece of tissue is slipped into the open end of the Fiberglass sleeving, which with its attached cover glass is then placed in a Kahn tube containing 0.7 ml of supernatant, which consists of 0.25 percent human serum protein in Hanks' balanced salt solution. The Kahn tube is closed with a sterile serum-type rubber stopper containing a No. 20 hypodermic needle (fitted ...

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Eleven pairs of identical twins, in which one had been previously hospitalized for schizophrenia or borderline schizophrenia, have been intensively studied, along with their parents, at the National Institutes of Health. A number of interacting features tend to consistently differentiate the life course of the index from the control twins.