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Dietary supplementation with antioxidants resulted in increased plasma concentrations of antioxidants. Moreover, supplementation decreased DNA oxidation and increased resistance of lipoprotein particles to in vitro oxidation. Antioxidant supplementation of sled dogs may attenuate exercise-induced oxidative damage.

Use of supplements containing the doses of antioxidants used here failed to attenuate exercise-induced increases in CK activity. Muscle damage in sled dogs, as measured by plasma CK activity, may be caused by a mechanism other than oxidant stress.

Exercise-associated hyponatremia occurs in horses and humans, both species that sweat, and in sled dogs, which do not sweat. To investigate the mechanism of exercise-associated hyponatremia in sled dogs, we measured water turnover, serum electrolyte concentrations and osmolality, plasma renal hormone concentrations, and urine composition of 12 fit Alaskan sled dogs before, during, and after a 490-km sled dog race (Ex group). Water turnover and serum electrolyte concentrations were measured in six similarly fit dogs that did not run (Sed group). Water turnover was significantly larger (P < 0.001) in Ex [190 +/- 19 (SD) ml . kg-1 . day-1] than in Sed dogs (51 +/- 13 ml . kg-1 . day-1). There were significant (P < 0.001) decreases in serum sodium concentration (from 148.6 +/- 2.8 to 139.7 +/- 1.9 mmol/l) and osmolality (from 306 +/- 9 to 296 +/- 5 mosmol/kgH2O) of Ex, but not Sed, dogs during the race. Plasma concentrations of arginine vasopressin decreased, whereas aldosterone and plasma renin activity increased significantly (P < 0. 01) during the race. Urine osmolality was unchanged, whereas urine sodium, potassium, and chloride concentrations decreased significantly (P < 0.05) and urine urea concentration increased (P = 0.06). These results demonstrate increased water turnover associated with hyponatremia and renal sodium conservation with maintained high urine osmolality in exercising Alaskan sled dogs.

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