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The tannin-protein-complex-degrading enterobacterium (T-PCDE), which specifically colonises the caecal wall of the koala, was investigated immunohistologically. Polyclonal antisera were raised against three strains of T-PCDE. Tissue from six koalas was subjected to immunogold staining using a pooled, absorbed antiserum. Numerous T-PCDE cells were observed in the bacterial layer attached to the caecal wall of five of the animals. The distribution pattern of T-PCDE varied: cells were either scattered throughout the bacterial layer or congregated in areas that apparently contained debris of digesta. The sixth animal, which had been treated with oxytetracycline and showed severe loss of body weight, did not have any bacterial layer attached to the caecal wall and did not stain positive for T-PCDE. The evidence suggests a symbiotic association between T-PCDE and the koala; the caecal wall and the wall of the proximal colon were 'strategically' colonised by the bacterium, which may facilitate access, although it is likely to be indirect, for the host animal to the nitrogen moiety liberated from breakdown of tannin-protein complexes.

A study has been made of the promoting effect of starch on cellulose digestion by mixed rumen bacteria in a cellulose-urea medium. Starch supplementation of the medium promoted the growth of bacteria that required neither amino acids (AA) nor branched-chain fatty acids (BrFA). The growth of these bacteria was followed by the growth of AA-dependent bacteria, AAor BrFA-dependent bacteria, BrFA-producing bacteria, and finally, BrFA-dependent cellulolytic bacteria. Population changes of these bacterial groups corresponded with a crossfeeding of AA and BrFA and the overall disappearance of cellulose. The data suggest that the nutritional interdependence among rumen bacteria affects the rate of cellulose digestion.

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