In a hospital in Lima, Peru, a review of 103 Staphylococcus aureus infections was conducted during 2002. The prevalence of oxacillin-resistant S. aureus strains was 68%; 25% of strains were resistant to multiple drugs. Previous use of antibiotics and undergoing a surgical procedure during the current hospital stay were associated with the presence of an oxacillin-resistant S. aureus strain.
HighlightsDeveloped a high-throughput microarray for anti-microbial susceptibility testing (AST).Demonstrated that the feasibility of the AST against clinical isolates of MRSA.Platform is a low sample volume, rapid, high-throughput alternative to traditional assays.
There is currently limited knowledge of the genome sequences of nonpathogenic Listeria species, especially strains from wildlife. Here, we report the draft genome sequence and associated genome information of an antibiotic-resistant Listeria innocua strain, UAM003-1A, isolated from the feces of a black bear in California, USA.
Overall, American adults have a 10.5% risk of having diabetes during theirlifetime but for the Hispanic American adult, the risk is over 12.5% comparedto 7.5% in non‐Hispanic whites. Hispanic Americans are a mixed groupcomprising of Mexicans, Cubans, Puerto Ricans, South and Central Americans,and other Spanish communities. This disease is very damaging and a challengeto manage for anyone. It is the seventh‐largest cause of death in the UnitedStates in 2017. Detecting single nucleotide polymorphism (SNPs), in thehuman genome can help in determining an individual's risk of obtaining type 2diabetes. Recent studies with SNP rs149483638 have shown that the IGF2gene is associated with the identification of insulin that regulates growth andmetabolism in the human body. IGF2 is found in many tissues where it issynthesized and released into the periphery, with the highest levels in theliver. IGF2 facilitates ß‐cell proliferation and survival in the pancreas. Thealleles shown in this SNP is the C>T allele change. The goal of this research wasto detect the prevalence of SNP rs149483638 by buccal swab samples in 25controls and 26 patients. DNA extraction followed by PCR and restrictionfragment length pattern using the BstNI enzyme was used to detect theprevalence of this SNP and its association with Diabetes. We found the allele C& T very predominant in the diabetes group. The allele C was also found in thecontrol group. We need to increase the sample size in the control group inorder to assess the risk of this SNP in diabetes type 2.
In Hispanics, type II diabetes is the fifth leading cause of death among Hispanics in the United States due to the genetic influence. Through this, scientists study the DNA to locate and study the specific DNA sequences called single nucleotide polymorphisms, SNP, associated with type II diabetes. The single nucleotide polymorphism, rs12255372, in recent studies has been associated with type II diabetes, breast cancer, and prostate cancer. The allele involve in the risk of Diabetes is G and T. DNA was extracted from the collected buccal samples. The 47 samples were run through a polymerase chain reaction (PCR) and confirmed by gel electrophoresis. After the amplification, the products were digested with the restriction enzyme MluCl. The G/G allele was the most predominant allele in both groups. Increasing sample size will allow for the data to be more concrete.
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