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Safe and effective laser ophthalmic surgery requires a fine balance between the efficiency of laser delivered and the degree of collateral side damage. The laser-ocular tissue interaction process is reliant on three main variables, namely, wavelength, pulse duration, and deposited energy. A certain amount of energy is needed to achieve ablation, while too much energy can result in unwanted collateral thermal damage. In our work the relationship between energy deposition and ablation effect is studied by an in-vitro experiment using an 800 nm wavelength 150 fs-pulse-duration laser system. This experiment aims to validate the probability of decreasing the supplied energy during glaucoma surgery by femtosecond laser. Our results show that less energy is needed using femtosecond laser than that using a longer pulse laser.