The aim of the present study was to investigate the sequence of shell bone formation in the embryos of the Pleurodira, Podocnemis unifilis. Their bones and cartilage were collected and cleared before staining. The shell was also examined by obtaining a series of histological slices. All the bony elements of the plastron have independent ossification centers, which subsequently join together and retain two fontanelles until the period of hatching. This turtle has a mesoplastra, which is characteristic of the Podocnemididae. The carapace begins to form concurrently with the ossification of the ribs at the beginning of stage 20. All the plates, except the suprapygal, initiate ossification during the embryonic period. The main purpose of the histological investigation was to highlight the relationship between the formation of the carapace and ribs with that of the neural plates. The costal and neural plates were found not to independent ossification centers, but to be closely related to components of the endoskeleton, originating as expansions of the perichondral collar of the ribs

ABTRACT.To gain an understanding of the detailed anatomical aspects of Mazama gouazoubira (brocket deer), this paper describes the relationships between its spinal cord and the vertebral canal, adding information with a clinical and surgical approach. Three specimens of M. gouazoubira were prepared following the methods normally used in anatomy. The epaxial muscles and vertebral arches were removed to expose the spinal cord and the spinal nerve roots. The dimensions of the medullary segments were measured using a pachymeter with 0.05 mm precision. The spinal cord is cylindroidal, dorsoventrally flattened, with an average craniosacral length of 656.27 mm, and has two dilatations corresponding to the cervical and lumbar intumescences. The cervical, thoracic, lumbar and sacrocaudal segments showed an average length of 175.07, 226.03, 123.47 and 43.63 mm, with indices of 28.02, 35.34, 19.68 and 6.93%, respectively. The medullary cone, whose average length is 46.27 mm, begins between L2 and L3 and ends between S1 and S2, with a mean index of 7.53%. The overall average distance between the nerve roots of the cervical, thoracic and lumbosacral segments was 2.23, 2.06 and 1.98 cm, respectively.Key words: brocket deer, neural system, skeletopy, spinal cord.RESUMO. Anatomia topográfica da medula espinal e relações vértebro-medulares em Mazama gouazoubira Fisher, 1814 (Artiodactyla; Cervidae). Propondo conhecer os aspectos anatômicos pormenorizados de Mazama gouazoubira (veado catingueiro), o presente trabalho descreve as relações entre sua medula espinal e o canal vertebral, adicionando informações com enfoque clínico-cirúrgico. Utilizaram-se três espécimes de M. gouazoubira que foram preparados seguindo métodos usuais em anatomia. Retirou-se a musculatura epiaxial e os arcos vertebrais para a exposição da medula espinal e raízes dos nervos espinais. As dimensões dos segmentos medulares foram obtidas utilizando um paquímetro de precisão 0,05 mm. A medula espinal possui a forma cilindróide, aplanada dorsoventralmente, com comprimento crânio-sacral médio de 656,27 mm, possui duas dilatações correspondentes às intumescências cervical e lombar. Os segmentos cervical, torácico, lombar e sacro-caudal apresentam 175,07; 226,03; 123,47 e 43,63 mm de comprimento médio, com índices de 28,02; 35,34; 19,68 e 6,93% respectivamente. O cone medular de comprimento médio 46,27 mm inicia-se entre L2 e L3 e termina em S1 e S2, com índice médio de 7,53%. A média geral obtida para a distância entre as raízes dos nervos dos segmentos cervical, torácico e lombossacral foi de 2,23; 2,06 e 1,98 cm, respectivamente.Palavras-chave: medula espinal, veado catingueiro, esqueletopia, sistema neural.

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Chemical restraint of large felids is necessary for the handling, transportation, clinical assessment, and induction of anesthesia. The objective of this study was to evaluate the efficacy and safety of chemical restraint using a combination of tiletamine, zolazepam, detomidine, and atropine with interspecific allometric scaling doses in captive Panthera onca. There were ten males (two melanistic ones) and five females weighing between 35 and 80 kg. The evaluation of the chemical restraint protocol was carried out during routine management of the animals for physical examination, collection of blood and bone marrow samples, dental evaluation, and other procedures. During pre-established intervals, rectal temperature, heart rate, respiratory rate, nociception of thoracic and pelvic limbs, muscle relaxation and loss of the righting reflex were monitored. Loss of the righting reflex occurred at 8 ± 1.89 minutes post-injection (MPI). Respiratory rate returned at 77.6 ± 8.18 MPI; return of consciousness occurred at 112.6 ± 12.28 MPI, and complete recovery at 153 ± 12.47 MPI. There were no statistical differences between heart and respiratory rate means and oxyhemoglobin saturation. For rectal temperature, there was a significant reduction. The protocol analyzed was efficient for the chemical restraint of jaguars, enabling the manipulation, transportation, physical examination, dental evaluation, blood and bone marrow sample collection, and other minor invasive procedures of medium duration in these animals.