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An initial report is made on the electrocardiogram of a single heart muscle cell in vivo. The On the basis of experience gained in a series of preliminary experiments, the tip of the microelectrode was assumed to lie in the interior of a relatively undamaged fiber when the record obtainecl showed the following characteristics:(1) a series of monophasic action potentials showing (2) overshoot and (3) no appreciable change of shape or form during the series; (4) a resting, potential of more than 50 millix olts;and (5)