The aim of this study was to evaluate the effects of different dietary inclusion of raw yellow lupine seed meal (YLM) on laying hen performance, the fatty acid (FA) profile, physicochemical, and sensory properties of eggs. A total of 224 Lohmann Brown laying hens at 32 wk age were fed isonitrogenous and isocaloric diets for 16 wk. The control diet contained soybean meal (SBM), and in study diets SBM was replaced with YLM at 100, 200, or 300 g/kg. In comparison with soybean, lupine seeds had a higher content of nonstarch polysaccharides (NSP) and raffinose family oligosaccharides (RFO) (29.5 vs. 14.0 and 8.56 vs. 5.91% DM). The dietary 300 g/kg lupine seeds increased the content of NSP and RFO in the ration, from 9.34 to 13.39 and 1.36 to 2.54%, respectively. The YLM inclusion level had no adverse effect on laying performance, including feed intake, FCR, egg production, and egg weight. The final BW of hens fed lupine-based diets were significantly higher compared with the control (P=0.039). Throughout the study, dietary treatments had no effect on eggshell and albumen quality. An increase in the inclusion rate of YLM was followed by a linear increase (P<0.001) in yolk color intensity. Dietary treatments had no influence on the aroma, taste, and texture of eggs evaluated in laying hens at 46 wk age. The inclusion of lupine seeds in experimental diets caused a linear increase in n-6 polyunsaturated FA (PUFA) content and the n-6/n-3 ratio (all P<0.001), but it had no influence on the atherogenic and the thrombogenic indices of egg yolk lipids. The results of this study indicate that YLM can be included at 300 g/kg in layer diets as a partial substitute for soybean meal without compromising laying performance, the physicochemical, and sensory properties of eggs.

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the aim of this study was to determine the effect of egg water loss during storage and incubation on hatch rates in heavy-type broad-breasted white but big 6 turkeys. turkey hens started laying eggs at 30 weeks of age. in weeks 2, 8, 16 and 21 of the laying season, 1512 eggs were selected randomly and divided into 4 groups of 378 eggs each. the groups of eggs were stored for 7, 10, 13 or 17 days before incubation. at the beginning and end of the storage period and on days 9, 15, 21 and 24 of incubation, eggs were weighed to determine the percent water loss relative to the egg's weight. four incubation cycles of 378 eggs each were performed for each storage period. a total of 16 incubation cycles were carried out (4 weeks of the laying season × 4 egg storage periods) and the following parameters were determined (%): egg fertilization, dead embryos, unhatched eggs and hatchability results from fertilized eggs. the percentages of dead embryos and unhatched poults with physical defects and abnormal position were determined in hatchery waste from each incubation cycle. Egg water loss varied throughout storage and reached 0.57% of total egg weight after 7 days, 0.79% after 10 days, 0.87% after 13 days and 1.28% after 17 days (P≤0.05). After 7 days of storage, egg water loss during a 15-day and 21-day incubation period reached 5.76% and 8.72%, and lower values were noted after 17 days of storage (P≤0.05). Egg water loss of 1.28% during storage resulted in a high rate of early embryonic mortality (14.81%) and a low hatch rate (72.12%) (P≤0.05). High water loss in stored eggs contributed to a higher percentage of congested embryos.