Background and Aim: Probiotics play an important role in maintaining a healthy gut and consequently promote good health. This study aimed to find novel probiotic lactic acid bacteria (LAB) from indigenous fermented foods of West Sumatera, Indonesia. Materials and Methods: This study utilized 10 LAB previously isolated from fermented buffalo milk (dadih), fermented fish (budu), and fermented cassava (tape) which have the ability to produce gamma-aminobutyric acid. The study commenced with the screening of LAB for certain properties, such as resistance to acid and bile salts, adhesion to mucosal surface, and antagonism against enteric pathogens (Escherichia coli, Salmonella Enteritidis, and Staphylococcus aureus). The promising isolates were identified through biochemical and gram staining methods. Results: All isolates in this study were potential novel probiotics. They survived at a pH level of 2.5 for 3 h (55.27-98.18%) and 6 h (50.98-84.91%). Survival in bile at a concentration of 0.3% was 39.90-58.61% and the survival rate was 28.38- 52.11% at a concentration of 0.5%. The inhibitory diameter ranged from 8.75 to 11.54 mm for E. coli, 7.02 to 13.42 mm for S. aureus, and 12.49 to 19.00 mm for S. Enteritidis. All the isolates (84.5-92%) exhibited the ability to adhere to mucosal surfaces. This study revealed that all the isolates were potential probiotics but N16 proved to be superior because it was viable at a pH level of 2 (84.91%) and it had a good survival rate in bile salts assay (55.07%). This isolate was identified as Lactobacillus spp., Gram-positive bacilli bacteria, and tested negative in both the catalase and oxidase tests. Conclusion: All the isolates in this study may be used as probiotics, with isolate N16 (Lactobacillus spp.) as the most promising novel probiotic for poultry applications based on its ability to inhibit pathogenic bacteria.

ABSTRAKPenelitian ini bertujuan untuk melihat persentase karkas, potongan komersial karkas dan lemak abdominal broiler dengan suplementasi andaliman (Zanthoxylum acanthopodium DC.) di dalam ransum. Penelitian menggunakan 80 ekor DOC broiler strain CP 707 produksi Chareon Pokhpan Indonesia. Ransum perlakuan diberikan umur 3 hari (bobot rata-rata 71.63±3.12 g/ekor) dipelihara sampai umur 30 hari. Penelitian menggunakan Rancangan Acak Lengkap dengan 4 perlakuan dan 4 ulangan dengan 5 ekor broiler setiap ulangan. Ransum perlakuan yang diberikan adalah: P1= ransum kontrol + 0% andaliman (Zanthoxylum acanthopodium DC.); P2= ransum kontrol + 0.25% andaliman (Zanthoxylum acanthopodium DC.); P3= ransum kontrol + 0.50% andaliman (Zanthoxylum acanthopodium DC.); P4= ransum kontrol + 0.75% andaliman (Zanthoxylum acanthopodium DC.). Peubah yang diamati adalah persentase karkas, persentase potongan karkas dan persentase lemak abdominal broiler. Hasil penelitian menunjukkan bahwa suplementasi andaliman di dalam ransum tidak berpengaruh nyata (P>0.05) terhadap persentase karkas dan potongan komersial karkas. Suplementasi andaliman sampai 0.75% menghasilkan persentase karkas yang lebih tinggi dibandingkan dengan ransum kontrol. Persentase karkas yang dihasilkan pada penelitian adalah 57.83±3.72% sampai 60.03±1.77%. Namun suplementasi andaliman dalam ransum berpengaruh nyata (P<0.05) terhadap persentase lemak abdominal. Persentase lemak abdominal penelitan adalah 0.46±0.06% sampai 0.83±0.06%. Kesimpulan hasil penelitian adalah suplementasi andaliman dalam ransum sampai 0.75% menghasilkan persentase karkas yang lebih tinggi dibandingkan dengan kontrol dan meningkatkan persentase lemak abdominal broiler. Kata Kunci: Andaliman (Zanthoxylum acanthopodium DC.), broiler, karkas, lemak abdominal. ABSTRACT This research aimed to seen the percentage of carcass, commercial carcass dan percentage abdominal fats of broiler with supplementation of andaliman (Zanthoxylum acanthopodium DC.) in the diet. The experiment used 80 day old chicken (CP 707 strain) production by Charoen Phokphan Indonesia. The experimental chicken was provided dietary when they were 3-days old (initial body wight of 71.63±3.12 g/bird) and terminated on day 30. A completely randomized design (CRD) with four treatment dan four replications and five birds in each replication was used in this experiment. Dietary treatment was: P1= Control diet + 0% andaliman (Zanthoxylum acanthopodium DC.); P2= control diet + 0.25% andaliman (Zanthoxylum acanthopodium DC.); P3= control diet + 0.50% andaliman (Zanthoxylum acanthopodium DC.); P4= control diet + 0.75% andaliman (Zanthoxylum acanthopodium DC.). variables measured were percentage of carcass, commercial carcass and percentage abdominal fats of broiler. The results showed that supplementation of andaliman (Zanthoxylum acanthopodium DC.) in the diet not significant (P>0.05) to the percentage of carcass dan commercial carcass. Supplementation andaliman of 0.75% produced the same percentage of carcass dan commercial carcass as than o...

This research aimed to increase functional value of broiler-chicken meat containing high antioxidant and low cholesterol through substitution of soybean meal (SBM) with Indigofera zollingeriana top leaf meal (ILM). The experiment used 160 day old broiler chicken (Cobb strain). The experimental chicken were provided dietary treatments when they were 15-days old (initial body weight of 460.5±1.56 g/bird) and terminated on day 35. A completely randomized design (CRD) with four treatments and four replications and ten birds in each replication was used in this experiment. Dietary treatments were: R1= diet containing 20% soybean meal (SBM) without I. zollingeriana top leaf meal (ILM); R2= diet containing 16% SBM and 5.9% ILM; R3= diet containing 12% SBM and 11.8% ILM; R4= diet containing 8% SBM and 17.74% ILM. Variables measured were performances (feed consumption, body weight gain, and feed conversion) and the quality of broiler meat (cholesterol, fat content, and malondialdehyde [MDA] concentration). The results showed that supplementation of 17.74% ILM (R4) as the substitution of 60% soybean meal protein produced the same performances of broilers as those of control diet (R1). Supplementation of 11.8% ILM as the substitution of 40% soybean meal protein (R3) decreased meat cholesterol by 34.70%, meat fat content by 52.93%, and MDA concentration by 62.52%. The conclusion of this study was that supplementation of 17.74% ILM produced the same performances as that of control diet, increased antioxidant content of the meat, indicated by a lower MDA concentration, and decreased cholesterol, as well as fat content of broiler-chicken meat.

The objectives of this research were to study the percentage of carcass of broiler and native chicken given arenga waste fermentation by Saccaromyces cerevisiae and to study arenga waste unfermentation in the ration. Sixty of broiler chickens Arbor Acres strain and sixty native chickens were divided into four dietary treatments and three replications (10 birds/replicate). The design of this experiment was completely randomized design factorial 2x2, with factor A was feeding (A1 : arenga waste unfermented, A2 : arenga waste fermented), and factor B was chicken species (B1:native chickens, B2:broilers). The variables observed were:  (1) body weight, (2) carcass, (3) percentage of carcass, and (4) Mortality.The purposes of this study  were to look at the effectiveness of rations and species of chicken used for the percentage of carcass and mortality. The results showed that there was no interaction (P> 0.05) between the fed arenga waste fermented or unfermented 2.5% in the ration,and  the type of chicken on weight, carcass and carcass percentage, but there was an interaction on mortality (P<0.01). The fed had no significant (P>0,05) effect on weight, carcass, and carcass percentage, but  percentage, but significant effect (P <0.05) on mortality.  Types of chickens had a significant effect (P <0.05) on weight, carcass, percentage  of carcass and mortality. The mortality rate with unfermented arenga waste was high enough, especially in broiler chickens, but it can be tolerated  on native chicken and broilers with saccaromyces cerviseae fermented arenga waste. Keywords : Arenga Waste, Fermentation Arenga Waste, Native Chickens, Broiler

The objectives of this research were to study the performances of broilers and native chicken given arenga waste (APAF) fermentation by Saccaromyces cerevisiae and arenga waste unfermentation (APA) in the ration. Sixty day-old chicks of broiler Arbor Acres strain and sixty native chicken were divided into four dietary treatments and three replications (10 birds/replicate). Design of this experiment was completely randomized design factorial 2x2, with factor A was feeding (A1 = arenga waste unfermentation, A2 = arenga waste fermentation), and factor B was chickens type (B1=native chicken, B2=broilers). The variables observed were: daily weight gain, feed consumption, and feed conversion. The results showed that the performances of the broiler given fed with unfermented and fermented arenga waste showing no significant differences between all treatments.  The results showed that there is an interaction between feeding and chicken type was no significant differences (P>0.05). Conclusion from this research of using fermented and unfermented arenga waste until level of 2.5% in ration showed no significant differences in the performance.