BACKGROUND: Inflammation occurs in chronic diseases such as type 2 diabetes mellitus (DM). Insulin resistance and inflammation in type 2 DM with obesity can increase interleukin (IL)-6, causing an increase in hepcidin synthesis in the liver. Increased inflammation can exacerbate the course of type 2 DM. This study aims to prove that there are differences in the levels of hepcidin and IL-6 between obese and non-obese type 2 DM.METHODS: This cross-sectional study was conducted on 61 patients with type 2 DM, consist of 22 male and 39 female with an age of more than 40 years. Type 2 DM subjects were obtained from a doctor's diagnosis and were divided into obese and non-obese groups based on body mass index (BMI). Hepcidin and IL-6 levels were examined using the Enzyme-Linked Immunosorbent Assay (ELISA) principle. The data were analyzed using an independent t-test and Mann-Whitney test.RESULTS: The mean level of hepcidin in the obese with type 2 DM group was 25.32±11.54 ng/mL, and non-obese was 11.94±5.31 ng/mL. The median level of IL-6 in the obese with type 2 DM group was 11.9 (5-61) pg/mL, and non-obese 4.8 (1.5-9.8) pg/mL. There was a significant difference in hepcidin and IL-6 levels between the obese and non-obese groups (p=0.000).CONCLUSION: Hepcidin and IL-6 levels in the obese group with type 2 DM were higher than non-obese group.KEYWORDS: type 2 diabetes mellitus, obesity, hepcidin, interleukin-6

Serum using is preferred for urea level because it does not use anticoagulants which can interfere with activity and  reaction to the results. The tubes that are widely used to collect blood into serum are  vacutainer serum separator and  vacutainer plain.This researche aims to determine the degree of agreement s between vacutainer serum separator and vacutainer plain usage on serum urea level result.This research was cross sectional design and hold on October 2020 with subject were taken from  thirty blood samples of health analyst students which taken randomly and had no history of disease or kidney function disorder. Each student was taken 6 ml of blood drawn using a venoject with each vacutainer containing 3 ml, so we had 60 data. The data were analyzed by descriptively and inferentially using the Interclass Correlation Coefficient (ICC) statistical test. From the descriptive analysis, the difference in mean levels was 0.35 mg/dL and the ICC statistical test resulted in a degree of agreement 0.745. The data were analyzed by descriptively and inferentially using the Interclass Correlation Coefficient (ICC) statistical test. From the descriptive analysis, the difference in mean levels was 0.35 mg/dL and the ICC statistical test resulted in a degree of agreement was 0.745. The calculation of the average working time between the vacutainer serum separator and the vacutainer plain was 4 minutes 38 seconds and 35 minutes 58 seconds. The analysis concluded that the vacutainer serum separator and the vacutainer plain could be used as an alternative of blood collecting tubes for urea level testing which proved to be no significant difference in the results from this research. Keywords : Urea level,  Vacutainer Serum Separator,  Vacutainer Plain

Background: Coronary heart disease (CHD) is caused by atherosclerosis in the coronary arteries. Lipid lipoprotein abnormalities as the pathogenesis basic of atherosclerosis can be assessed using atherogenic index of plasma (AIP) and oxidized low density lipoprotein level (ox-LDL). Troponin I has a role in heart muscle damage due to ischemia in CHD. This study aimed to determine the differences of AIP, ox-LDL and troponin I levels in patients with stabel CHD and acute coronary syndrome (ACS).Methods: This is an observational analytic study with cross-sectional including 34 stable CHD patients and 34 ACS patients from January to March 2019 at Kariadi hospital Semarang. Measurement of triglycerides (TG) and HDL using automatic chemistry, AIP is calculated from log TG/HDL, ox-LDL levels using the ELISA method, troponin I using the ELFA method. AIP statistical analysis used independent t-test while ox-LDL and troponin I levels statistical analysis was using Mann whitney U test. Significant difference was determiner when p < 0.05.Results: Mean ± SD of AIP in stable CHD was 0.52 ±  0.25 and ACS was 0.55 ± 0.23 with  p = 0.622. Median (min-max) of ox-LDL in stable CHD was 324.74 pg/ml (67.44 – 891.98) and ACS was 717.18 pg/ml (87.35 – 1959.4) with p £ 0.001 and median (min – max) troponin I in stable CHD was 0.026 ug/L (0.018 – 0.035) and ACS was 1.5 ug/L (0.003 – 40) with p £ 0.001.Conclusion: There were significant differences in ox-LDL and troponin I levels between stable CHD and ACS. There was no significant differences ofAIP show in stable CHD patients and ACS. Pendahuluan: Penyakit jantung koroner (PJK) disebabkan aterosklerosis pada arteri koroner. Kelainan lipid lipoprotein yang menjadi dasar patogenesis aterosklerosis dapat dinilai dengan atherogenic index of plasma (AIP), oxidized low density lipoprotein (ox-LDL). Troponin I berperan pada kerusakan otot jantung akibat iskemia pada PJK. Tujuan dari penelitian ini adalah untuk membuktikan perbedaan nilai AIP, kadar ox-LDL dan troponin I pada pasien PJK stabil dan sindrom koroner akut (SKA).Metode: Penelitian observasional analitik dengan pendekatan potong lintang dilakukan pada 34 pasien PJK stabil dan 34 pasien SKA di RSUP Dr. Kariadi Semarang. Penetapan trigliserida (TG) dan HDL menggunakan kimia otomatik, AIP dihitung dari log TG/HDL, kadar ox-LDL menggunakan metode ELISA, troponin I mengunakan metode ELFA. Analisis statistik AIP menggunakan independent t-test sedangkan kadar ox-LDL dan troponin I menggunakan Mann whitney U test. Perbedaan bermakna apabila p < 0.05.Hasil: Rerata ± SD nilai AIP PJK stabil adalah 0.52 ± 0.25 dan SKA 0.55 ± 0.23 dengan p = 0.622. Median (min – maks) ox-LDL PJK stabil 324.74 (67.44 – 891.98) pg/ml dan SKA 717.18 (87.35 – 1959.4) pg/ml dengan nilai p £ 0.001, dan median (min – maks) troponin I PJK stabil 0.026 (0.018 – 0.035) ug/L dan SKA 1.5 (0.003 – 40) ug/L dengan nilai p £ 0.001.Simpulan: Terdapat perbedaan bermakna kadar ox-LDL dan troponin I dan perbedaan tidak bermakna nilai AIP pada pasien PJK stabil dan SKA.

Kanker kolorektal merupakan salah satu dari kejadian kanker tersering di Indonesia. Penyebab dari kanker kolorektal sendiri sampai saat ini belum diketahui secara pasti, tetapi banyak faktor risiko yang dipercaya dapat menyebabkan kanker kolorektal, salah satunya adalah jumlah asupan serat dari sumber makanan sehari-hari seperti sayur, buah, dan kacang-kacangan. Beberapa peneliti yang menemukan adanya korelasi signifikan terhadap diet dan kejadian kanker kolorektal berspekulasi bahwa makanan yang kita makan akan mempengaruhi keberagaman mikrobiota yang berada di dalam usus yang nantinya akan mempengaruhi proses terbentuknya kanker. Selain itu terdapat kecenderungan bahwa anak-anak jaman sekarang kurang menyukai sayuran dan lebih menyukai makanan instan yang rendah serat. Selain sebagai sumber serat, sayuran juga merupakan sumber vitamin dan mineral yang penting untuk tubuh. Kegiatan pengabdian masyarakat ini merupakan aplikasi dari health promotion dengan cara menginisiasi agar kurikulum sekolah dasar dapat terkandung materi terkait konsumsi sayuran, sehingga anak-anak didik menjadi terbiasa mengkonsumsi sumber serat terutama sayuran. Pengabdian kepada masyarakat ini berlokasi di empat sekolah di wilayah Kabupaten Sleman. Pemilihan sekolah didasarkan pada letak geografis dan kedekatan akses makanan siap saji. Kegiatan ini meliputi pengukuran pengetahuan tentang kanker kolon, tentang pentingnya konsumsi dan pola konsumsi sayuran pada siswa SD sasaran. Setelah itu diikuti dengan pemberian materi dalam bentuk audio-visual dan buku “Ayo Makan Sayuran”, kemudian dilakukan pengukuran peningkatan pengetahuan dan motivasi konsumsi sayuran pada siswa SD sasaran.

Background : Egg  is one of the animal protein source, which has delicious taste, easy to digest and highly nutritious. Besides its affordable price, its supply availability is unquestionable as well. However, due to its short storability, it requires special treatment, such as preserving, to store it for long period. One way to preserve the egg is by pickling egg, which generally requires seven to ten days of marinating. During the process of marinating, there will be a visual change of egg white and yolk. Their structures  will be more solid (the occurrence of thickening process) because salinization will lead to protein denaturalization. Consequently, it has an influence as well towards the content of egg white protein of duck egg. This study is aimed to explore the impact of various time of pickling egg towards egg white protein of duck egg. Method  : The study where takes place in a laboratories, is a true experimental study for the reason that the researcher must provide intervention, hence all of potentially confounding variables are manageable. Samples that had been used in this study are duck eggs which were bought from North Brebes. This study is expected to generate data from four various time of pickling egg and control (no treatment). Since there are four samples, accordingly the number of data resulted are twenty. The resulted data will be descriptively presented in table, graph, presentation, and narration. Result  : Protein level examination within duck white egg shows changes  in protein levels that occurs in every variation of pickling egg time, where the average results of the assay of duck egg white protein is 14.94% without treatment (control), in five days of pickling time is 13.68%, in seven days of pickling time is 13.29%, in nine days of pickling time is 12.87% and eleven days of pickling time is 12.78%. Conclusion  : There is a significant impact among the period of pickling time to the protein level degradation of duck white egg. Keywords : Duck egg, period of pickling time, level protein of duck white egg.