Cara sitasi: Diachanty S, Nurjanah, Abdullah A. 2017. Aktivitas antioksidan berbagai jenis rumput laut coklat dari Perairan Kepulauan Seribu. Jurnal Pengolahan Hasil Perikanan Indonesia. 20(2): 305-318. AbstrakRumput laut cokelat mengandung berbagai metabolit (karotenoid, laminarin, alginat, fukoidan, manitol dan florotanin) yang berfungsi sebagai bahan anti-kanker, antioksidan dan agen kemopreventif berbagai penyakit degeneratif. Penelitian ini bertujuan untuk menentukan komposisi kimia dan aktivitas antioksidan dari rumput laut cokelat Sargassum polycystum, Padina minor dan Turbinaria conoides. Penelitian terdiri atas analisis proksimat, serat kasar, mineral, logam berat, ekstraksi, fitokimia, total fenol dan aktivitas antioksidan dengan metode DPPH, FRAP dan CUPRAC. Hasil penelitian menunjukkan S. polycystum, P. minor dan T. conoides mengandung kadar air 24-31%, lemak 0,23-0,47%, protein 3-4%, abu 27-45%, karbohidrat 26-38% dan serat kasar 2-6%. Kandungan mineral Fe S. polycystum, P. minor dan T. conoides, yaitu 0,03 g/kg hingga 0,39 g/kg, Ca 11,28 g/kg hingga 27,98 g/kg, K 13,86 g/kg hingga 41,15 g/ kg, Na 16,31 g/kg hingga 20,51 g/kg dan Mg 8,04 g/kg hingga 18,68 g/kg. Logam berat Pb S. polycystum, P. minor dan T. conoides yaitu 3,48 mg/kg hingga 12,68 mg/kg, Hg 0,24 mg/kg hingga 0,53 mg/kg dan Cu 5,66 mg/kg hingga 7,02 mg/kg. Ekstrak etanol S. polycystum, P. minor dan T. conoides memiliki senyawa alkaloid, fenolik, flavonoid, saponin steroid dan triterpenoid. Kandungan total fenol ketiga rumput laut cokelat yaitu 3758,97-8287,18 mg GAE/g. Ketiga rumput laut cokelat tersebut memiliki aktivitas antioksidan DPPH dengan nilai IC 50 1,9-9,6 mg/mL, FRAP 70,643-105,357 µmol troloks/g dan CUPRAC 85,268-201 µmol troloks/g. Kata kunci: CUPRAC, DPPH, FRAP, fenolik, rumput laut cokelat Antioxidant Activities of Various Brown Seaweeds from Seribu IslandsAbstract Brown seaweeds contain various metabolites (carotenoids, laminarin, alginate, fucoidan, mannitol, and phlorotannin) as anticancer substances, antioxidants and chemopreventive agent against degenerative diseases. This research was aimed to determine the chemical properties and antioxidants activity of Sargassum polycystum, Padina minor and Turbinaria conoides. This research consisted of the proximate analysis, crude fiber, minerals, heavy metals, extraction, phytochemicals, analysis phenolic compound and antioxidant activity with DPPH, FRAP and CUPRAC methods. The results showed moisture content of S. polycystum, P. minor, and T. conoides 24-31%, fat 0.23-0,47%, protein 3-4%, ash 27-45%, carbohydrate 26-38% and crude fiber 2-6%. Mineral Fe S. polycystum, P.minor and T.conoides about 0.03 g/kg to 0.39 g/kg, Ca 11.28 g/kg to 27.98 g/kg, K 13.86 g/kg to 41.15 g/kg, Na 16.31 g/kg to 20.51 g/kg and Mg 8.04 g/ kg to 18.68 g/kg. The range of heavy metals Pb S. polycystum, P. minor and T. conoides 3.48 mg/kg to 12.68 mg/kg, Hg 0.24 mg/kg to 0.53 mg/kg and Cu 5.66 mg/kg to 7.02 mg/kg. Ethanol extract of S. polycystum, P. minor, and T. conoides contains alkaloids, phenoli...

Seaweed extract has ability to reduce some effects of free radicals because of phenolic compound. The purpose of this study was to determine total phenolic content, total flavonoids and antioxidant activity of seaweed extract from Turbinaria conoides and Eucheuma cottonii with different solvents. The extraction was carried out by n-hexane (non-polar) solvent, ethyl acetate (semi-polar) and methanol (polar). The results showed that the highest total phenol and flavonoids were found in  extract of Turbinaria conoides with ethyl acetate 211.00 mg GAE/g and 157.16 mg QE/g. Seaweed Eucheuma cottonii extract using methanol solvent had a higher phenol content 141.00 mg GAE/g, whereas the highest flavonoid content found in ethyl acetate solvent (35.1771 mg QE/g). The very strong antioxidant activity (< 50 μg/mL) was found in Turbinaria conoides and Eucheuma cottonii which extracted by methanol solvent (15.148 μg/mL and 23.154 μg/mL). Seaweed Turbinaria conoides and Eucheuma cottonii had a great potential as natural antioxidant.

Diversification of traditional food ilabulo made from raw catfish (Pangasius sp.) has the potential to be developed in Gorontalo province to substitute chicken viscera. The research aimed to make ilabulo substitute the raw material of chicken viscera with the catfish fortified Kappaphycus alvarezii seaweed and catfish bone meal and to determine the organoleptic and chemical characteristics of ilabulo catfish fortification. Fortified treatment is K. alvarezii  and catfish bone (5 dan 10% ),  B (10 and 15%) and  C (15 dan 20%). The organoleptic analysis used a hedonic scale of favorite criteria on the appearance, texture, color, flavor, and taste. The results of organoleptic analysis continued with Bayes test. The chemical analysis used the Association of Official Analytical Chemist method. The result of the hedonic characteristic of ilabulo catfish fortification was on the appearance  neutral criteria – like (5.53–7.03), texture neutral criteria – rather like (5.8–7.1), aroma rather like (6.3–6.73), color neutral – like (6.1–7.03) and taste (6.07–6.53) neutral criteria – rather like. The result of Bayes test obtained by a texture of importance value 5, appearance importance value 5, aroma of importance value 4, color of interest value 3 and taste of importance value 2. Characteristic of ilabulo culture of selected fortified catfish that was the fortification of K. alvarezii seaweed 15% and catfish  bone flour (20%) (fortification C) obtained by water content 56.46%, ash 11.54%, protein 7.78%, fat 8.91%, coarse fiber 0.61%, carbohydrate 22.07% and calcium 0.315 %.

<p>Plants lindur (Bruguiera gymnorrhiza) is one of the plants that have a potential as<br />a source of bioactive compounds for the antioxidant. This study aimed to determine the<br />chemical composition, bioactive compound and determine antioxidant activity of leaves,<br />bark and roots of plants lindur (B. gymnorrhiza). This research was conducted through<br />several stages proximate test, stratified extraction, phytochemical test, and antioxidant<br />activity test with DPPH. The highest yield value resulting by ethanol extract of the leaves<br />is 12,85 % and the lowest was produced by n-hexane root extract that is 0,18 %. The leaves<br />contain high levels of protein, fat, and water which is the higher of the two parts from the<br />other samples. Bark containing ash content (4,12%) and carbohydrates (46,02%). Root has<br />the highest levels of carbohydrates that is 25,91 %. Bioactive components were detected on<br />ethanol and ethyl acetate extracts of the leaves are flavonoids, tannins, phenols, saponins,<br />steroids and tritepenoid. Bark extract ethanol, ethyl acetate and ethanol root bark has<br />bioactive components there are flavonoids, tannins, phenols, saponins, and tritepenoid. </p><p>Ethyl acetate extract of the roots only have bioactive components there are flavonoids,<br />phenols, saponins, and triterpenoids. The potential antioxidant activity produced from<br />ethyl acetate extracts of bark lindur with IC50 values is 14,21 ppm.<br />Keywords: Antioxidants, phytochemicals, roots, ethanol, Bruguiera gymnorrhiza</p>

Cara sitasi: Abdullah A, Nurjanah, Reyhan M. 2017. Karakterisasi dan identifikasi senyawa aktif ekstrak pigmen telur keong mas. Jurnal Pengolahan Hasil Perikanan Indonesia. 20(2): 286-295. AbstrakKeong mas (Pomacea canaliculata) diketahui menjadi hama bagi tanaman padi karena kemampuan adaptasi dan daya reproduksi yang tinggi. Salah satu upaya untuk memberantas hama keong mas dengan memanfaatkan telur keong mas untuk bahan baku produk dalam bidang industri pangan maupun kesehatan. Tujuan penelitian ini yaitu mengidentifikasi senyawa aktif yang terkandung dalam ekstrak pigmen telur keong mas. Metode penelitian yang digunakan meliputi ekstraksi pigmen menggunakan aseton dan metanol, analisis metabolit sekunder, dan penentuan senyawa aktif secara semi kuantitatif dengan LC-MS/ MS. Rendemen ekstrak pigmrn telur keong mas yaitu 33,80% pada pelarut aseton dan 36,10% pada pelarut metanol. Hasil identifikasi pada ekstrak metanol pigmen telur keong mas menunjukkan adanya senyawa aktif berupa pigmen yaitu 11 pigmen karotenoid golongan xantofil, 2 pigmen karotenoid golongan karoten, dan 2 senyawa aktif berupa non pigmen, sedangkan pada ekstrak aseton menghasilkan 11 pigmen karotenid golongan xantofil dan 2 senyawa aktif berupa non pigmen. Kata kunci: karotenoid, LC-MS/MS, komponen aktif, Pomacea canaliculata Identification and Profiling of Active Compounds from Golden AppleSnail's Egg Pigments Abstract Golden apple snail (Pomacea canaliculata) has been known as a pest for rice plant due to high adaptability and reproductive power. Utilization of Pomacea canaliculata's eggs as raw materials in the food and health industry is one of the efforts to eradicate the pest snail. This study was aimed to identify the active compounds contained in the extract pigments of Pomacea canaliculata's eggs. The methods of this study were an extraction of pigments using acetone and methanol, analyzing the active compound (secondary metabolite) qualitatively, TLC to determine pigment components and LC-MS/MS to identify active compounds semi quantitatively. The yield of Pomacea canaliculata's eggs pigment extracts is 33,80% in acetone solvent and 36,10% in methanol solvent. The results showed that active compounds in the methanol extract contain 11 carotenoid pigments of a xanthophyl group, two carotenoid pigments of carotene group, and 2 active compounds in non-pigmented form, whereas the acetone extract contains 11 pigment carotenoids of a xanthophyl group and 2 compounds active in non-pigment form. Keywords: active ingredients, carotenoids, LC-MS/MS, Pomacea canaliculata