No abstract

The study aims at the Fourier spectra of an antibiotic exposed to a high-intensity pulsed magnetic field. The applied frequency was f = 40 kHz, with a number of pulses n = 1, at intensities of the pulsed magnetic field H = 0.09 × 106 А/m, Н = 0.50 × 106 А/m, Н = 0.82 × 106 А/m. It was assumed that under the conditions of the pulsed magnetic field there might be a change in the structure of the drug molecule. The research has revealed that an increase in the pulsed magnetic field power leads first to a decrease in the intensity of the band in the Fourier spectrum, and then to its increase. In addition, changes were recorded in the amide group of benzylpenicillin sodium salt molecules depending on the intensity of the pulsed magnetic field applied to the antibiotic.

Pulsed magnetic fields are widely used in various fields of modern technology. In our previous works it was shown the strengthening of antibacterial properties of benzylpenicillin sodium salt after exposure to a pulsed magnetic field of high tension. The reason for this may be a change in the conformation of β-lactam antibiotics under external physical influence, which increases the affinity of benzylpenicillin molecules to the active center of penicillin-binding proteins. This article presents studies on the safety of benzylpenicillin sodium salt irradiated by a pulsed magnetic field. Benzylpenicillin sodium salt in powdered form was irradiated with a pulsed magnetic field with the intensity H = (0.09∙106-1.23∙106) A/m, frequency f = 30-70 kHz, and a different number of pulses (n = 1-3), using inductors – single-turn and multi-turn. The influence of pulsed magnetic field on chemiluminescence parameters of irradiated benzylpenicillin sodium salt was studied. The appearance of free radicals in irradiated preparations was evaluated in simple model systems simulating the most common free radical oxidation reactions in the body and in environments in which the formation of reactive oxygen species and lipid peroxidation reactions was initiated. Acute toxicity in intraperitoneal administration of antibiotic to mice before and after treatment with pulsed electromagnetic field with calculation and comparison of LD50 indices was also investigated. It was found that the treatment of the dry substance benzylpenicillin sodium salt by a pulsed magnetic field does not cause the formation of free radicals and is not accompanied by an increase in the toxicity of this antibiotic. The possibility of using new ways to increase the antibacterial activity of antibiotics without increasing their concentrations is considered.

The paper studied the effect of high-intensity pulsed magnetic fields (PMF) on extraction of humic acids in order to increase the yield of the suitable product. It is proposed to add to the technological chain of reactions for the precipitation of humic acids the operation of PMF processing of raw materials at the stage of the brawn coal suspension in alkali (the first experiment) or the alkaline extraction suspension (the second experiment).Pulse-magnetic processing the brawn coal suspension in alkali and the alkaline extraction suspension was carried out at magnetic field intensities H=0.09 106 A/m and H=0.82 106 A/m with the frequency of the discharge circuit f=40 kHz and the number of pulses n=1. The experiments have shown that when humic acids are obtained by water-alkaline extraction followed by their precipitation in an acid medium, for the highest yield of the product it is necessary to add the operation of pulse-magnetic processing a raw material at the magnetic field intensity of 0.82 106 A/m at the stage of the brawn coal suspension in alkali. It can be assumed that a high-intensity PMF contributes to the transfer of humic acids to a state conducive to their more efficient precipitation and extraction.