In this work we introduce a novel microfluidic enzyme linked immunoassays (ELISA) microplate as the next generation assay platform for unparalleled assay performances. A combination of microfluidic technology with standard SBS-configured 96-well microplate architecture, in the form of microfluidic microplate technology, allows for the improvement of ELISA workflows, conservation of samples and reagents, improved reaction kinetics, and the ability to improve the sensitivity of the assay by multiple analyte loading. This paper presents the design and characterization of the microfluidic microplate, and its application in ELISA.

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Miniaturized, high throughput detection technologies including microfluidics systems represent powerful tools for biomarker discovery and analysis. Optimiser™ microplate technology combines microfluidics technology with standard SBS-configured 96-well microplate architecture and allows for the improvement of ELISA workflows. In this review, we present the "standard" and "high sensitivity" capabilities of the Optimiser™ ELISA in detecting human cytokine biomarker; IL-4, resulting in improving sensitivity by 1000-fold higher than the typical conventional ELISA. The Optimiser™ ELISA microplate employs standard ELISA equipment and protocols, selectively reduces sample volume 10-20 fold (Static Mode) and has the capability to amplify assay sensitivity by 1,000 fold (Repetitive Loading Flow-Through Mode) relative to conventional high sensitivity ELISA approaches. Optimiser™ allows sensitive and quantitative detection of biomarkers with demonstrated reproducibility, speed and linearity. In this study we demonstrate the utilization of Optmiser TM in the amplification of low-concentration markers. Optimiser™ microfluidicbased technology represents a revolutionary advancement in ELISA technology, and holds great promise for accurate, sensitive detection of novel biomarkers and its potential applications in clinical diagnosis.

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