BACKGROUND: Population aging is considered to be a global phenomenon today. Age-associated immune system dysfunction or “immunosenescence” is indicated by increased susceptibility to infections and chronic diseases, such as hypertension, diabetes mellitus, autoimmune diseases, heart disease, and atherosclerosis. One of the immunosenescence markers is a significant drop in CD28 and reduced proinflammatory cytokine interleukin-2 (IL-2). The mango mistletoes are deemed to have a better affinity for docking the CD28 and IL-2R receptors of α and β subunits than other plants. AIM: This study aims to determine the effect of ethanol extract of mango mistletoes on IL-2 levels, the percentage of CD4+CD28+, and the percentage of CD8+CD28+ in aged female mice. METHODS: The leaves of mango mistletoes were extracted using 96% ethanol solvent, and the extract was administered to aged female mice (18–20 months) orally with different doses for each group, namely 150, 300, and 600 mg/kg. Mango mistletoe leaves extract was administered once a day for 14 days. Then, the IL-2 levels of the mice were checked from their heart blood samples using Enzyme-Linked Immunosorbent Assay, while the percentages of CD4+CD28+ and CD8+CD28+ were examined from the spleen samples using flow cytometry. RESULTS: The ethanol extract of mango mistletoe leaves was able to increase the percentage of CD4+CD28+ significantly (p < 0.05) at doses of 300 and 600 mg/kg and increase the percentage of CD8+CD28+ significantly (p < 0.05) at a dose of 600 mg/kgBW, while other various doses had a strong enough correlation (r = 0.48) to increase IL-2 levels. CONCLUSION: The ethanol extract of mango mistletoe leaves has the good potential to inhibit the aging process in the immune system, as characterized by an increase in IL-2 levels and the percentage of CD4+CD28+ and CD8+CD28+.

Estrogen has anti-oxidative and anti-inflammatory properties, but its levels decrease in postmenopausal women who can trigger oxidative stress. One of the most damaging effects of ROS is lipid peroxidation, and the end product is Malondialdehyde (MDA). Similarly, aging endothelium has increased oxidative stress and endothelial cell sensitivity to apoptosis. This study aimed to determine the effect of cowpea extract on serum MDA levels, aortic endothelial cell counts, and brain MDA levels in the ovariectomy model. Cowpea extract can be used as an alternative to prevent and overcome the effects that occur during menopause, such as cardiovascular problems, decreased bone mineral density, and dementia. The study used 15-month-old female Rattus norvegicus, divided into six groups (OVX, SHAM, OVX+estradiol, OVX+Vu 1.25; 2.5; and 5 mg/kg BW/day). Serum and brain MDA levels were examined by ELISA method, while the number of aortic endothelial cells were examined on histopathological preparations with Hematoxylin & Eosin (HE) staining. The mean value of serum and brain MDA levels decreased with an increase in the dose given (p-value 0.016). The mean value of aortic endothelial cells between the dose groups did not significantly differ. However, the mean value showed an increasing trend as the dose of cowpea extract was given. The results of this study indicate that the extract of cowpea has the potential as an antioxidant to reduce serum and brain MDA levels, prevent a decrease in the number of aortic endothelial cells. As prevention, cowpea extract can be used as an antioxidant and consumed since premenopause to minimize problems that occur during postmenopause.

Stunting is a form of malnutrition caused by inadequate food intake, repeated infections, and lack of psychosocial stimulation. It has an impact on growth and development disorders but is more accurately referred to as undernutrition. In this regard, the role of the mother is important and problems that occur during pregnancy are very likely to contribute to stunting. This study aimed to describe stunting and the grande multipara phenomenon from a gender perspective. A qualitative approach was used to explore in-depth information about the behavior of pregnant women, parity and exclusive breastfeeding at Panglima Sebaya Hospital in July 2019. Based on the results of interviews with pregnant women, most knew about exclusive breastfeeding because health workers often provided counseling to mothers during and after pregnancy. However, mothers with multiple parity / grande multipara had difficulty carrying out exclusive breastfeeding because the time between the children was close. The village midwife informant said that many pregnant women have used family planning, especially the millennial generation; they have been exposed to a lot of media including social media, and many are independent, can decide for themselves to use family planning and choose the method of family planning. Stunting and grande multipara are closely related to gender inequality in the domestic environment. Keywords: stunting, grande multipara, gender, breastfeeding

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Introduction: Polycystic Ovary Syndrome (PCOS) is the most common causes of infertility in reproductive age women. The lack of knowledge about this disease causes PCOS is often late diagnosed. This study aims to determine the influence of health promotion in video-based learning form on young women’s knowledge, attitudes and behaviors in early screening efforts for PCOS. Methods: This is a pre-experimental study with one-group pretest-posttest design. The sample used was 261 female students in one of the senior high schools in Denpasar with a simple random sampling technique. The research instrument used is a questionnaire in which each variable, namely the knowledge, attitude, and the behavioral. The data was analyzed by using the Wilcoxon Rank Test. Results: There was a significant change between the provision of health promotion in the form of video-based learning form on young women’s knowledge, attitudes and behaviors related to the PCOS early screening in each variable, i.e. the knowledge variable p-value = 0.001, the attitude variable p-value <0.001, and the behavior variable p-value <0.001. Conclusion: There was an influence of health promotion in video-based learning form on young women’s knowledge, attitudes and behaviors related to the PCOS early screening.