The effects of kinds of lumus and the storage period on the quality of patin wadi based on the results of nutrient tests AIP Conference Proceedings 1844, 030003 (2017); https://doi.org/10.1063/1.4983430Mitochondrial DNA pattern of the fine shrimp Metapenaeus elegans (De Man, 1907) Abstract. Bacteriocin is a peptide that is easily degraded by proteolytic enzymes in the digestive systems of animals, including humans. It has antimicrobial activity against pathogenic bacteria. Lactobacillus sp. is one type of lactic acid bacteria (LAB) that occupies the intestines of ducks (Anas domesticus L.). The purpose of this research was to determine the optimum time of the highest protein production by Lactobacillus sp. and to determine inhibitory activity of bacteriocin against pathogenic bacteria (Escherichia coli and Staphylococcus aureus). Using the Bradford method, the results showed that the optimum time of highest bacteriocin production was after 36 hours of incubation, with a protein content of 0.93 mg/ml. The bacteriocin inhibitory activity against Escherichia coli showed that a protein concentration of 30% gave a maximum inhibition index of 1.1 mm, while for Staphylococcus aureus, a concentration of 70% gave a maximum inhibition index of 0.3 mm. Further research is required to determine the stationary state of bacteriocin production in this circumstance.
Rhizosphere bacteria have the ability to protect the host plants from the infection of pathogenic microorganisms. This study aimed to identify rhizosphere bacteria that were capable of inhibiting the growth of bacterial isolates that cause tuber rot of Amorphophallus muelleri. Rhizosphere bacteria were isolated using Nutrient Agar medium by pour plate method. Isolates were subjected to antagonistic assay against several bacterial isolates from the rotten tuber of A. muelleri using dual culture method. The potential isolate was identified based on 16S rDNA sequence. Isolate R7 showed the strongest inhibition to the growth of bacterial isolates from rotten tuber with an inhibition zone diameter of 19.66 mm. The 16S rDNA sequence of isolate R7 R7 was 99.7% similar to Delftia tsuruhatensis PCL1755. The isolate was potential to be developed as phytopathogen control agent.
Tuberkulosis paru adalah salah satu penyakit infeksi menular yang menyerang saluran pernapasan bawah yang disebabkan oleh bakteri patogen yaitu Mycobacterium tuberculosis. Kesulitan dalam penanganan tuberkulosis salah satunya disebabkan oleh terjadinya resistensi antibiotik rifampisin sebagai antibiotik lini pertama. Resistensi rifampisin dikendalikan oleh gen rpoB (RNA Polymerase β-Subunit) pada Mycobacterium tuberkulosis yang akan terekspresi ketika terjadi mutasi. Penelitian ini bertujuan untuk mengetahui mutasi gen rpoB pada penderita tuberkulosis paru di BBKPM Makassar. Penelitian ini merupakan penelitian deskriptif dengan desain cross sectional menggunakan 20 sampel sputum positif tuberkulosis yang telah melakukan terapi antibiotik lini pertama selama dua bulan. Deteksi mutasi gen rpoB dengan metode PCR dan dilanjutkan dengan sekuensing. Hasil dari penelitian yaitu didapatkan mutasi gen rpoB pada 17 sampel, yang terjadi pada posisi 1304 bp, 1322 bp, 1333 bp, 1348 bp, 1349 bp dan 1471 bp. Kesimpulan dari penelitian ini yaitu terjadinya mutasi gen rpoB resisten terhadap antibiotik rifampisin.
Demam tifoid adalah penyakit menular yang menimbulkan infeksi akut pada usus kecil dan disebabkan oleh Salmonella typhi. Terapi tifoid adalah dengan pemberian antibiotik. Penggunaan antibiotik yang tidak rasional menyebabkan peningkatan bakteri yang resisten. ESBL adalah enzim pada Salmonella sehingga bakteri tersebut memiliki mekanisme resistensi terhadap antibiotik seperti golongan sefalosporin generasi ketiga. Salah satu antibiotic golongan sefalosporin yang banyak digunakan adalah cefotaksim dan ceftriakson. Tujuan penelitian ini adalah untuk mengetahui uji sensitivitas S. typhi terhadap antibiotik sefalosforin generasi ketiga pada penderita demam tifoid. Sampel yang digunakan sebanyak 43 isolat S. typhi penderita demam tifoid. Hasil penelitian menunjukkan dari 43 sampel isolate yang digunakan, hanya 4 isolat yang diidentifikasi sebagai S. typhi. Uji sensitivitas 4 isolat S. typhi menunjukkan bahwa keempat sampel tersebut sensitif terhadap antibiotik cefotaksim dan ceftriakson dengan zona hambat terluas yaitu pada kode sampel H.36 dengan antibiotik cefotaksim sebesar 29,5 mm dan antibiotik ceftriakson 38,5 mm.
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