The development of a DNA-vaccine against classical swine fever (CSF) is a perspective direction, because it gives an opportunity to develop a marker vaccine due to use of a part of protective antigen molecule, and to induce effectively both cellular and humoral immune response. In this study a recombinant plasmid, containing the fragment of E2 gene of CFS virus (CSFV) in eukaryotic expression vector, has been developed. It has been demonstrated that the fragment of E2 protein of CSFV is expressed in CHO-K1 cells from the developed recombinant plasmid pTR-BKneo -, and we suggest that the protein possesses the post-translational modifications. The data obtained are in favor of the created model DNA-vaccine able to induce humoral immune response to fragment of E2 protein of CSFV.

e object of the paper is to show the heterogeneity of 300 cord samples processed in the current research. e differences in effectiveness of mesenchymal stem cell (MSC) isolation are shown. Moreover, the recommendations for choosing the method of MSC isolation depending on the value of stromal-vascular rate are given. e data can be useful for selecting the optimal conditions to obtain MSC and for further cryopreservation of umbilical cord tissue.

Розробка ДНК-вакцин є одним з найперспективніишх напрямків у сучасній вакцинології. Проте, незважаючи на швидкі темпи розвитку цього напрямку в прикладному аспекті, недостатньо вивченим залишається широке коло питань щодо базових механізмів індукції імунної відповіді при застосуванні зазначеного методу імунізації. З огляду на це створено модельну ДНК-вакцину, де як ген модельного антигену використано ген ß-галактозидази Е. coli. У результаті експериментів показано, що створена модельна рекомбінантна плазміда pBG-TR/02 здатна індукувати про дукцію специфічних до ß-галактозидази Е. coli антитіл у мишей залежно від місця її введення, тобто може бути використана як модельна ДИК-вакцина у подальших дослідженнях. Ключові слова-ДНК-вакцина, модельний антиген, імунізація, гуморальна імунна відповідь.

Aim. The classic detachment techniques lead to changes in cells properties. We offer a simple method of cultivating the population of cells that avoided an influence on the surface structures. Methods. Mesenchymal stem cells (MSC) from human umbilical cord matrix were obtained and cultivated in standard conditions. While substituting the culture media by a fresh portion, the conditioned culture medium, where the cells were maintained for three days, was transferred to other culture flacks with addition of serum and growth factors. Results. In the flacks, one day after medium transfer, we observed attached cells with typical MSC morphology. The cultures originated from these cells had the same rate of surface markers expression and clonogenic potential as those replated by standard methods. Conclusions. MSC culture, derived by preserving the cells with reduced attachment ability, actually has the properties of «parent» passage. Using this method with accepted techniques of cells reseeding would allow maintaining the cells that avoided an impact on the cell surface proteins