Cold atmospheric plasma has been developed and utilized as a novel technique for skin rejuvenation because of its various effects on cells and living things. This study investigated the accuracy of this claim and any possible side effects of using spark plasma to rejuvenate skin. The present work is the first quantitative investigation using animal models. 12 Wistar rats were divided into two groups for this investigation. To compare the skin's natural process with the treated skin, the first group underwent a single session of plasma therapy, while the second group served as the control group. The back of the necks of the samples was shaved for 20 cm. Before beginning treatment, the MPA9 multifunctional skin tester was used to determine the melanin index, erythema index, and transepidermal water loss (TEWL). The skin's thickness and density were assessed using sonography, and its elasticity index was calculated using a Cutometer. The samples were exposed to plasma radiation in the designated area (in a triangular pattern). The abovementioned signs were examined immediately after the following therapy and at the weekly appointment 2–4 weeks later. Optical spectroscopy was also used to demonstrate the presence of active species. In this study, we found that a plasma spark therapy session significantly boosts skin elasticity, and the ultrasound results revealed a significantly increased skin thickness and density. The plasma increased the amount of skin surface evaporation, erythema, and melanin immediately following the treatment. However, 4 weeks later, it recovered to its former state and did not differ significantly from before the therapy.

Cold atmospheric plasma has been developed and utilized as a novel technique for skin rejuvenation because of its various effects on cells and living things. This study investigated the accuracy of this claim and any possible side effects of using spark plasma to rejuvenate skin. The present work is the first quantitative investigation using animal models. 12 Wistar rats were divided into two groups for this investigation. To compare the skin's natural process with the treated skin, the first group underwent a single session of plasma therapy, while the second group served as the control group. The back of the necks of the samples was shaved for 20 cm. Before beginning treatment, the MPA9 multifunctional skin tester was used to determine the melanin index, erythema index, and transepidermal water loss (TEWL). The skin's thickness and density were assessed using sonography, and its elasticity index was calculated using a Cutometer. The samples were exposed to plasma radiation in the designated area (in a triangular pattern). The signs mentioned above were examined immediately after the following therapy and at the weekly appointment, 2-4 weeks later. Optical spectroscopy was also used to demonstrate the presence of active species.

Beauty and youth are the most critical and challenging issues in medicine and beauty science, and humans always seek to find a new method for the elixir of youth. Innovative technologies based on physical principles have also been presented in recent years. In the meantime, cold plasma technology, which previously showed exciting results in the skin, is currently being investigated due to its remarkable ability to treat diseases and skin care. This study used the FEDBD plasma device for skin rejuvenation in animal samples. There were two groups of six male Wistar rats. One group was treated with plasma with a power of 3.3 W, and the second group was considered the control group for a better comparison. Plasma processing was done on the back of the neck of mice with an area of 18 square centimeters. So far, complete quantitative measurement has not been done, and few studies have been done. Before starting the treatment, immediately after the treatment, the fourth week, and the tenth week of follow-up, biometric tests were performed, including moisture level, evaporation from the skin surface, erythema and melanin, skin elasticity and firmness with an MPA9 device and cutometer. The thickness and density of the epidermis and dermis, an essential indicator in rejuvenation, were evaluated with a skin ultrasound device. Also, the level of oxygen, perfusion, and interstitial water (edema) was checked using a Tivita tissue hyperspectral camera at a depth of 6 mm of the skin. Active species produced by plasma, plasma temperature, and device power were investigated and recorded using physical tests.

The skin is the body's largest organ and protects against mechanical, thermal, and physical harm. However, the skin's function and appearance decrease visibly with age due to repeated exposure to adverse environmental impacts such as UV rays and toxic substances and the development of oxidative stress in aging. During aging, these factors cause phenotypic changes in the skin, such as wrinkles, pigmentation, loss of elasticity, and moisture. SPARK plasma has been found in studies to preserve skin against aging effectively. The effects of two separate spark plasma device modes with voltages of 3.44 and 4.88 kV on skin health, including pigmentation, skin firmness, elasticity, and moisture loss, were studied using the MPA9 multifunctional skin tester in this study. In addition, utilizing ultrasound, the effectiveness of two plasma modes on skin density and thickness was examined. The findings of this study suggest that spark plasma may slow the skin's aging process by enhancing its elasticity, density, and thickness. However, there were no difficulties or scars at the treatment location at the end of this study, indicating that Spark plasma can be utilized as a new way to delay the aging process.