Background: Cancer is one of the leading causes of death worldwide, therefore struggles to find more effective treatment and prevention is needed. Several studies has been performed using natural ingredients, one of which is Temu Kunci (B. pandurata). Temu Kunci extract contains flavonoid pinostrobin that has been showed as having cytotoxicity effects. Cytotoxicity tests of pinostrobin have been performed on several tumor cell lines, but its cytotoxicity effect on HeLa cell line has never been reported. Objective: To assess cytotoxicity effect of pinostrobin temu kunci on HeLa cell culture Methods: This study used simple experimental design. Pinostrobin were isolated from temu kunci and proved by TLC densitometry compared to standard pinostrobin. HeLa cell culture were treated with pinostrobin with concentrations 5, 25, 50, 75, 100, and 250 ug/mL. Cytotoxicity test were performed by MTT assay. Data were analyzed using one-way ANOVA. Results: There was significant difference (p=0.000) of means of cell viability percentage, respectively: 92.58 ± 9.84 (5µg/mL), 91.78 ± 4.4 (25µg/mL), 80.09 ± 4.51 (50µg/mL), 76.89 ± 7.75 (75µg/mL), 67.85 ± 11.31 (100µg/mL), dan 48.82 ± 16.61 (250µg/mL). The IC50 was 250µg/mL. Conclusion: Pinostrobin showed no active cytotoxicity effect on HeLa cell culture. Latar Belakang: Penyakit kanker merupakan salah satu penyebab morbiditas dan mortalitas yang tinggi hampir di seluruh dunia, sehingga diperlukan berbagai upaya untuk pencarian pengobatan dan pencegahan yang lebih efektif. Beberapa penelitian dilakukan dengan menggunakan bahan yang berasal dari alam. Salah satu bahan alam Indonesia yang banyak diteliti adalah tumbuhan rimpang Temu Kunci (B. pandurata). Ekstrak tumbuhan tersebut mengandung antioksidan flavonoidpinostrobin yang diketahui memiliki daya toksisitas terhadap sel kanker, tetapi belum diketahui bagaimana daya sitotoksisitasnya terhadap kultur sel HeLa. Tujuan: Untuk mengkaji daya sitotoksik pinostrobin temu kunci terhadap kultur sel HeLa. Metode: Penelitian ini menggunaan desain eksperimental murni. Pinostrobin diisolasi dari rimpang temukunci dan dibuktikan dengan analisis densitometri TLC dibandingkan dengan

BB cream merupakan istilah dari blemish balm, blemish base, beblesh balm dan beauty balm di pasar barat yang dirancang menjadi alas bedak, pelembab dan tabir surya sekaligus. Penggunaan BB cream diduga berhubungan bermakna dengan keparahan klinis akne vulgaris, karena penggunaan kosmetik ini dapat meningkatkan sebum akibat unsur komedogenik yang terkandung di dalamnya, seperti: lanolin, petrolatum, butyl stearate, lauryl alcohol dan oleic acid. Kandungan parfum seperti linalool, limonene, caffeine, geraniol, dan citral yang terkandung dalam BB cream juga dapat menyebabkan reaksi alergi dan iritasi sehingga meningkatkan risiko untuk terjadinya akne vulgaris. Penelitian ini bertujuan untuk mengetahui potensi efek komedogenik dan iritasi akibat pemberian produk kosmetik BB cream pada telinga kelinci. Penelitian ini menggunakan desain true experimental laboratory dengan metode randomized post test only controlled group design. Penelitian ini menggunakan hewan coba kelinci New Zealand white jantan. Dilakukan aplikasi topikal 3 produk BB cream pada telinga kanan selama lima minggu sedangkan telinga kiri berfungsi sebagai kontrol negatif. Efek komedogenik dan iritasi dinilai berdasarkan skoring. Hasil uji statistik dengan menggunakan uji Fisher exact didapatkan nilai p= 1,00 (p>0,05), oleh karena itu disimpulkan bahwa tidak terdapat hubungan bermakna antara aplikasi topikal BB cream dengan pembentukan komedo dan perubahan iritasi pada kulit telinga kelinci.

An understanding of the socio-demographic characteristics is the first step to conduct preventive and promotive measures to reduce the number of sexually transmitted infections. This study described the socio-demographic characteristics of gonorrhea and syphilis in dr. Soedono Hospital Madiun. A descripttive study with a quantitative approach used secondary data from outpatient medical records. This research was conducted in dr. Soedono Hospital Madiun, East Java. The sam-ples used in this study were 61 patients who had been diagnosed with gonorrhea and syphilis in the period from July 2013 -June 2016. The data consisted of 50 (82%) medical records with the diagnosis of gonorrhea and 11 (18%) medical records with a diagnosis of syphilis. Characteristics of patients/ respondents are the highest age group of 21-30 years, 22 female patients (36.1%), 55 male patients (90.2%), unmarried status 33 people (54.1%), work as private employees 37 people (60.7%), pay without insurance 48 people (78.7%), and live around Madiun 52 (86.0%). The socio-demographic factors were dominant in patients with gonorrhea and syphilis in dr. Soedono Hospital period of 2013-2016 was 21-30 years old, male, unmarried, living as private sector employees, pay without insurance, and Madiun origin.