The increasing incidence of antimicrobial resistance is a public health concern, and Pseudomonas aeruginosa is known to be resistant to a variety of antibiotics. Antimicrobial resistance and the multiple antibiotic resistance (MAR) index of P. aeruginosa from environmental and clinical sources were studied in the current study. A total of 170 samples were evaluated, with 85 samples each from environmental sources and clinical settings. The isolates were subjected to microbial analysis and antimicrobial sensitivity testing. The findings revealed that 45.88 % (39) of the 85 clinical isolates tested for the presence of P. aeruginosa were positive. In terms of prevalence, there were significant variations (p 0.05) between the clinical samples. Wound samples had the highest isolation rate of 28.2%, while urine samples had the lowest (12.8%). P. aeruginosa was found in 38.8 % (33/85) of the samples isolated from environmental sources. In terms of prevalence, there was a highly significant difference (p 0.01) between the isolates. All of the positive isolates were completely resistant to cefuroxime and amoxicillin (100 %). The majority were also resistant to, cotrimoxazole (82%), nalidixic acid (82%), ciprofloxacin (86%), and tobramycin (69%). There was a substantial variation in the resistance patterns of isolates. The current study demand comprehensive measure to combat antimicrobial resistance in P. aeruginosa.

Industrial waste is mainly responsible for accumulating Zn (II) in the soil, which needs to be removed to avoid its bioaccumulation and hazardous effects on the environment. In a recent study, the potential of the RY12 strain was evaluated as a biosorbent of Zn (II) ions in an aqueous medium. Different microbiological techniques like biochemical, molecular characterization, and 16S rRNA gene sequencing were used for the identification of RY12. The impact of different parameters such as the initial zinc ion concentration, pH, temperature, and the removal of other metals such as manganese, lead, cobalt, silver, copper, mercury, and chromium was also evaluated on the reduction of Zn (II). Fourier Transform Infrared spectroscopy (FTIR) was also carried out to investigate the role of cellular surfaces in the sorption of Zn+2 ions. Both biochemical and phylogenetic analyses established that strain RY12 Pseudomonas sp. capable of reducing Zn+2 up to 89% at 28°C (pH = 6.5; initial Zn+2 concentration = 200 mg/L). The FTIR analysis revealed that the bacterial cell wall’s amino, carboxyl, and phosphate groups were involved in the reaction with Zn (II). Our findings suggest that Pseudomonas sp. RY12 is a proficient bacterium for removing zinc from industrial waste and could be a valuable bioremediation agent.

During the previous few years, the relationship between the gut microbiota, metabolic disorders, and diet has come to light, especially due to the understanding of the mechanisms that particularly link the gut microbiota with obesity in animal models and clinical trials. Research has led to the understanding that the responses of individuals to dietary inputs vary remarkably therefore no single diet can be suggested to every individual. The variations are attributed to differences in the microbiome and host characteristics. In general, it is believed that the immanent nature of host-derived factors makes them difficult to modulate. However, diet can more easily shape the microbiome, potentially influencing human physiology through modulation of digestion, absorption, mucosal immune response, and the availability of bioactive compounds. Thus, diet could be useful to influence the physiology of the host, as well as to ameliorate various disorders. In the present study, we have described recent developments in understanding the disparities of gut microbiota populations between individuals and the primary role of diet-microbiota interactions in modulating human physiology. A deeper understanding of these relationships can be useful for proposing personalized nutrition strategies and nutrition-based therapeutic interventions to improve human health.

Objective: To determine the effects of proprioception training in improving balance in patients with diabetic neuropathy. Methods: A quasi-experimental design study was conducted at the Safi Hospital Faisalabad for eight weeks from August to December 2019. Out of, 38, 19 patients were placed in exercise group (10 males, 9 females; mean age 64 ± 7.7 years; range 60 to 83 years) and 19 patients were placed in controls group (12 males, 7 females; mean age 63 ± 8.2 years) were included in this study through consecutive sampling technique. Proprioception Training was given to exercise group twice a week for eight weeks and the diabetes awareness campaign was given once a week to the control group. Static and dynamic balance were assessed by using One Leg Standing (OLS) Test with eyes open and closed, Berg Balance Scale (BBS), Functional Reach Test (FRT), Timed Up and Go Test (TUGT) and 10-M Walk Test (10-MWT). The data was collected before and after treatment and was compared using independent sample t-test. Results: The finding of the study showed that OLS score with eyes open improved significantly with p<0.05 and does not show improvement with eyes closed p =.073. The dynamic balance from the Berg Balance Scale, Functional Reach Test, Timed Up and Go Test and 10-M Walk Test revealed significant improvement after the balance exercises with p<0.05 Conclusion: It is concluded that proprioception training exercises are effective in improving balance among patients with Diabetic neuropathy. Continuous...

COVID-19 pandemic has affected millions of individuals globally over the last three years and is spreading continuously. In view of different studies and clinical findings, patients suffering with COVID-19 frequently have deranged liver function tests (LFTs), but the clinical significance of this finding is debatable. Objective: The present study was aimed to estimate the prevalence, features, and clinical significance of deranged LFTs in COVID-19 infected individuals, who were hospitalized but were not critically ill. Method: We conducted a cross sectional from May 2021 to December 2021 at The Akbar Niazi Teaching Hospital Islamabd. A total of 250 COVID-19 patients were included in the current study. The patient's blood samples were collected to get laboratory results, which included LFTs. LFTs were performed at the time of admission and every 5 ± 2 day throughout the stay. The outcome measure was either death or transfer of the patients to an intensive care unit. Results: At the time of admission, 160 patients (64%) showed deranged LFTs. Individuals with deranged LFTs experienced more severe inflammation, swelling, and organ damage than those who didn’t. Patients with deranged LFTs had a greater proportion of transfer to the ICU (81 vs 17), hospital stay (17 vs 7 days), and death (17 vs 5) than those with normal LFTs. Conclusion: The results of the current investigation demonstrated that LFTs data might forecast the degree of illness in patients with COVID-19 infections at the time of admission and during their hospital stay. Keywords: Covid-19, Liver Function Tests, ICU, Mortality Rate, Pakistan