In this study, 39 specimens belonging to Lespedeza species (Lespedeza cyrtobotrya, L. bicolor, L. maximowiczii, and Lespedeza cuneata) (Leguminosae) were classified phenotypically and genotypically. We constructed a phylogenetic tree based on the combined nrDNA (internal transcribed spacer; ITS) and cpDNA (trnL-trnF) sequences with the aim of classifying the genotypes. Samples were mainly divided into three genotypes. Samples of L. cyrtobotrya and L. bicolor were mixed in a single branch, whereas samples of L. maximowiczii and L. cuneata were clustered within species, respectively. We performed a liquid chromatography-electrospray ionization-mass spectrometry-based metabolite profiling analysis to classify the phenotypes. Multivariate statistical analyses such as principal component analysis (PCA) and hierarchical clustering analysis (HCA) were used for the clustering pattern analysis and distance analysis between species, respectively. According to the PCA and HCA results, leaves were classified into four phenotypes according to species. In both the genetic and chemotaxonomic classification methods, the distance between L. cyrtobotrya and L. bicolor was the closest between species, and L. cuneata was the farthest away from the other three species. Additionally, orthogonal partial least squares-discriminant analysis was employed to identify significantly different phytochemicals between species. We classified L. cyrtobotrya and L. bicolor by identifying significantly different phytochemicals. Interestingly, leaves and stems showed different phenotypic classifications based on the chemotaxonomic classification. Stem samples of the other three species were mixed regardless of species, whereas L. cyrtobotrya stem samples were clustered within species. The phenotypic classification of leaves coincided more with the genotypic classification than that of stems. Key message We classified four wild-type Lespedeza sp. by analyzing the combined nrDNA (ITS) and cpDNA (trnL-trnF) sequences. We also classified leaves and stems of Lespedeza sp. by applying liquid chromatography-mass spectroscopy-based metabolite profiling.
In a CuxO solid-electrolyte-based CBRAM cell using an Ag top electrode, electroforming-free and electro-reset processes could be achieved at a specific ex situ annealing temperature of the solid electrolyte.
Abstract-The inverse synthetic aperture radar (ISAR) image can be very effective in target recognition because it provides 2-D image that uses frequency data measured at various observation angles. However, the jet engine modulation (JEM) that can occur in the received signal due to the rotation of the blade in the engine may result in image blurring in cross-range direction. In this paper, we propose an efficient method of removing JEM signals by using the existing chirplet basis function and an efficient method to estimate the initial values of the four parameters of the chirplet. Simulations using the measured data provided clear ISAR image of a real Boeing747 aircraft.
Dilleniaceae) is described from HonBa Nature Reserve of the Khanh Hoa province of Vietnam. Detailed illustrations and taxonomic comments are provided along with a table listing the differential characters to the closely similar taxa. Phylogenetic analyses using nrITS region of ribosomal DNA and psbA-trnH intergenic spacer region of chloroplast DNA sequences were also performed and they supported the status of D. tetrapetala as a distinct species. The plant is considered endangered based on the IUCN red list criteria because of its restricted distribution.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.