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Additional index words. Diospyros kaki, inheritance, tanninAbstract. Pollination-constant and nonastringent (PCNA) is one of the most desirable traits in persimmons as this type of cultivar loses its astringency while still on the tree before harvest. Among Japanese PCNA cultivars, the trait is qualitatively inherited and recessive to pollination-constant, astringent (PCA), pollination-variant, nonastringent (PVNA), and pollination-variant, astringent (PVA) types. However, in a previous trial, both astringent and nonastringent types segregated in the F 1 population that resulted from a cross between a Chinese PCNA 'Luo Tian Tian Shi' and a Japanese PCNA cultivar. Because of the unusual segregation, in this study, we crossed another Japanese PCNA 'Okugosho' with 'Luo Tian Tian Shi' to confi rm the segregation of astringent types by measuring the tannin cell size and tannin concentration at harvest. Previously, we found that astringent types have larger tannin cells than PCNA-type. The F 1 hybrid progenies from the cross segregated into both PCNA and astringent-type individuals in approximately 1:1 ratio. Likewise, the F 1 population from the astringent-type 'Yotsumizo' and 'Iwasedo' × 'Luo Tian Tian Shi' were ascertained to contain both PCNA and astringent types, which indicates that the PCNA trait of 'Luo Tian Tian Shi' was dominant. Thus, this Chinese cultivar has the potential to become an important parental material for future breeding of PCNA persimmons.

Working machines in construction sites or emergency scenarios can operate in situations that can be dangerous to an operator both\ud for direct control of the machine or teleoperation in co-presence. Remote operation has been typically hindered by limited sense\ud of presence of the operator in the remote of the environment to due the reduced field of view of cameras. Starting from these\ud considerations we are introducing a novel real-time panoramic telepresence system for construction machines. This system does\ud allow fully immersive operations in critical scenarios while keeping the operator in a safe location at moderate distance from the\ud construction site. An omnidirectional stereo vision head mounted over the machine acquires and streams data to the operator with a\ud streaming technique that focuses on the current direction of site of the operator. According to the motion of the operators head, the\ud telepresence system will determine the involved cameras to use and the appropriate region of interest in cameras. The operator uses\ud a head-mounted display to experience the remote site also with the possibility to view digital information overlaid to the remote\ud scene as a type of augmented reality. The paper addresses the design and architecture of the system starting from the vision system\ud and then proceeding to the immersive visualizatio

Persimmon cultivars are classified into four types depending on the nature of astringency loss of the fruit, namely pollination constant non-astringent (PCNA), pollination variant non-astringent (PVNA), pollination variant astringent (PVA), and pollination constant astringent (PCA). Among these four types, PCNA is the most important cultivar for persimmon breeding due to the stable loss of natural astringency on the tree. The trait of natural astringency loss is recessive in Japanese PCNA cultivars, while that in the Chinese PCNA cultivar, 'Luo tian tian shi', is dominant and the latter locus, termed CPCNA, is different from that in Japanese cultivars. In order to develop a molecular marker for the selection of the CPCNA-type cultivar, we performed amplified fragment length polymorphism (AFLP) in combination with bulked segregant analysis for F 1 offspring derived from 'Luo tian tian shi', which was used as the maternal parent. A total of 384 primer combinations were tested, and three AFLP markers, namely EACT-MCCC-222 (RO1), EGGC-MCTC-309 (RO2), and EGCC-MCGA-105 (RO3), linked to the CPCNA dominant allele were obtained. Among these markers, EGGC/MCTC-309 (RO2) was converted into a sequence-characterized amplified region (SCAR) marker. PCR analysis using F 1 offspring (n = 264) revealed that the relevance ratio of the SCAR marker was 94%. The polymorphism of the RO2 marker, which was strongly linked to the CPCNA dominant allele, was detected in only two Chinese PCNA cultivars, namely 'Luo tian tian shi' and 'Tian bao gai', among the Chinese, Korean, and Japanese cultivars tested. These results indicate that the RO2 marker contributes to marker-assisted selection for breeding programs of new PCNA cultivars having the CPCNA trait.

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