Article rec ¸u le 07 novembre 2019, accept é le 03 mars 2020 Résumé. L'étape préanalytique de l'examen cytobactériologique des urines (ECBU) est l'une des plus critiques en microbiologie. Les objectifs de notre étude étaient de déterminer le taux de contamination des urines et de décortiquer les facteurs qui favoriseraient ceci pour proposer des solutions à cette problématique. Méthode : C'est une étude descriptive de 26 mois incluant toutes les demandes d'ECBU adressées à notre laboratoire. Les urines étaient traitées conformément aux recommandations du référentiel en microbiologie médicale. Les urines étaient considérées comme contaminées en cas de culture polymorphe à au moins trois différents types de germes avec un dénombrement à partir 10 3 UFC/mL. Résultats : Nous avons colligé 16 412 demandes d'ECBU. Les urines étaient contaminées dans 4 830 cas (29,43 %). Parmi les urines contaminées, 39,23 % (n = 1 895) émanaient des services des urgences, 79,44 % (n = 3 837) étaient prélevées au milieu du jet, 69,83 % (n = 3 373) émanaient d'une patiente de sexe féminin et 16,34 % (n = 789) provenaient d'enfants âgés de moins de 5 ans. Discussion et conclusion : Pour réduire la contamination des urines, des dispositions écrites décrivant les modalités de prélèvement devraient être disponibles et les prélèvements au milieu du jet et à travers les sacs adhésifs collecteurs devraient être remplacés par les prélèvements par ponction sus-pubienne chez les enfants chaque fois que le rapport bénéfice/risque de cette méthode est favorable.

Background and Purpose:The presence of yeasts in the urine is not synonymous with urinary tract infectionsinceit can result insimple colonization or contamination. Regarding this, it is required to further clarify the epidemiological profile of funguria. Accordingly, the present study was conducted to establish the epidemiology of funguriainthe Mohammed VI Teaching Hospital of Oujda, Morocco. Materials and Methods:This retrospective studywas conducted onall urine samples sent for cytobacteriological examination to amicrobiology laboratoryover a period of 28 months(i.e., from March 2016 to June 2018). After the removal of duplicates, the urinesampleswere treated according to the recommendations of the medical microbiology standards. Results:A total of15,165 urine sampleswerecollected. Urinary colonization accounted for 4.94% (n=749) of cases. The infections of the urinary tract accounted for 5.35% (n=811) of cases. Microbial isolates (n=1,669) in colonization and urinary tract infections were dominated by bacteria (93.47%, n=1,560). Furthermore, the yeasts accounted for 6.53% (n=109) of the isolates. Candidaalbicanswas isolated from56.88% (n=62) of funguriacases. Theriskfactors forfunguriain our series wereessentially old age, admission tointensive care unit, and broad-spectrum antibiotic therapy. Conclusion:The current level of knowledge about the clinical situations leading to funguria with the improvement and popularization of efficient identification techniques for yeasts other than C. albicans should redress the epidemiology of funguria.This should allow the knowledgeable societies to establish the rules of interpreting the cytobacteriological examination of the urine in case of funguria, as for bacteriuria.

Background The aim of this work is to present the results of a comparative study between the ALT assay on Integra 400 plus Roche Diagnostic versus Architect ci8200 of Abbott Diagnostic. Methods A total of 200 patients hospitalized in the various departments of the university hospital Mohammed VI of Oujda were prospectively tested on two systems: Abbott Architect ci8200 and Roche Integra 400 plus. Both analyzers use the spectrophotometric technique by coupling the transamination reaction to the oxidation-reduction reaction at NAD. The agreement of the results between the different techniques was evaluated using the Bland-Altman difference diagram and the Passing-Bablok and Deming regression line. Results There was a high concordance between the two assays: the equation of the Passing-Bablok line is YArchitect ​= ​−0,5625 ​+ ​0,9917 XIntegra with a correlation coefficient r 2 = 0.999. The Bland-Altman diagram shows that the mean bias between the two methods is of the order of 1.1 IU/L and the difference between the ALT measurements by Architect and Integra is in the range of −1.4 to 3.6. Conclusion Our study shows a high correlation of the ALT assay results between the architect ci8200 and Integra 400 plus.

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