Pluchea lanceolata is an important medicinal plant of Asteraceae family known for its anti-arthritic and anti-inflammatory activity. A protocol was established for micropropagation of P. lanceolata using nodal explants. Nodal explants were inoculated onto Murashige and Skoog (1962) - MS medium supple–mented with 6-benzylaminopurine (BAP), kinetin (Kin), thidiazuron (TDZ) and 2iP (2-isopentenyladenine) at various concentrations (0.0, 0.5, 1.0, 1.5 and 2.0 mg·dm-3). The highest multiplication rate was obtained for nodal explants cultured on MS medium, supplemented with 0.5 mg·dm-3 thidiazuron (TDZ). In vitro raised shoots were successfully rooted on ½ mineral salt concentration of MS medium supplemented with 1.0 mg dm-3 IBA.

Withania coagulans (Stocks) Dunal is an important medicinal plant of Solanaceae. Nodal segments obtained from field grown plants were used as explants. 1 -2 cm long nodal segment with a single one bud was cultured on MS containing 2.5 mg/l thidiazuron (TDZ), 0.1 mg/l NAA and 50 mg/l adenine sulphate (AdS) resulted in formation of 5.16 shoots per node. However, vitrification was observed in all explants within one month. On the other hand nodal explants cultured on MS supplemented with 2.50 mg/l meta-topoline (mT) with 0.1 mg/l NAA and 50 mg/l AdS resulted in the formation of 4.50 healthy and uniformly grown shoots per node.