<p class="A-ISIABSTRAK">A study was conducted to explore the bioactive substances of some local plants in order to find their effectiveness as antioxidant, antibacteria and antifungi to be used as feed additives. Twelve plants material were used in this study. The total phenol, tannin and saponin contents in the plant extract were assayed. The extracts were also assayed on their antioxidant activities and on their ability to depress in vitro gas production of microbes obtained from chicken’s guts, their ability to inhibit growth of bacteria (E. coli and Salmonella enteridis) and fungi (A. niger). The results showed that the highest total phenol and total tannin contents were found in clove leaf extract, while the highest saponin content was found in Sapindus rarak fruit pericarp. The highest antioxidant activity was found in the leaffruit extract. Gas produced by microorganims was reduced to the level similar to antibiotic addition were found with addition of hexane- extract of leaffruit, kapok seed or methanol- extract of mangosteen fruit rind pulp or clove leaves. The best inhibitory effect on E. coli growth (measured by clearing zone) was found in methanol extract of S. rarak fruit. However, the most effective growth inhibitor for both E. coli and Salmonella was the liquid smoke of cashew nut shell. The best growth inhibitor for fungal growth was found in extract of clove leaves. Therefore, clove leaves extract (anti fungi), liquid smoke of cashew nut shell (antibacteria) and leaffruit (antioxidant) may have potential to produce feed additives to substitute antibiotic growth promoters.</p>

ABSTRAKHidayat C, Iskandar S, Sartika T , Wardhani T. 2016. Respon pertumbuhan galur ayam lokal terseleksi terhadap ransum dengan kadar energi metabolis dan protein berbeda. JITV 21(3): 174-181. DOI: http://dx.doi.org/10. 14334/jitv.v21i3.1397 Penelitian ini bertujuan untuk mengetahui respon pertumbuhan galur ayam lokal hasil seleksi terhadap ransum dengan kadar energi metabolis dan protein ransum berbeda.. Tiga kelompok anak ayam hasil perkawinan galur ayam lokal terseleksi, yaitu Line 1 (♂KUB dengan ♀KUB), Line 2 (♂SenSi dengan ♀KUB) dan Line 3 (♂Gaok dengan ♀KUB), diberi tiga ransum perlakuan berbeda, yang terdiri dari ransum Diet 1 (2800 kkal ME/ kg dengan 17,81% protein kasar), Diet 2, (2950 kkal ME/ kg dengan 18,61% protein kasar) dan Diet 3 (3100 kkal ME/ kg dengan 19,25% protein kasar). Percobaan ini menggunakan rancangan percobaan faktorial 3x3. Setiap kombinasi perlakuan diulang sebanyak 7 kali dengan jumlah anak ayam umur sehari (DOC) sebanyak 5 ekor/ulangan, yang dipelihara sampai dengan umur 10 minggu. Hasil penelitian menunjukkan bahwa galur Line 2 (♂SenSi x ♀KUB) menunjukkan bobot hidup umur 10 minggu yang lebih tinggi (P<0,05), feed conversion ratio (FCR) yang rendah dan European Production Efficiency Factor (EPEF) yang tinggi, dibandingkan dengan kedua galur lainnya. Ransum yang optimum untuk galur Line 2 adalah ransum Diet 2, maka diambil kesimpulan bahwai galur ayam hasil perkawinan ♂SenSi dengan ♀KUB berpotensi untuk dimanfaatkan sebagai ayam lokal tipe pedaging komersial di Indonesia dengan menggunakan ransum optimum yang mempunyai kadar energi 2950 kcal ME/kg dengan 18,61% protein kasar. The aim of doing this experiment was to observe the growth response of improved native breeds of chicken under diets differed in energy and protein content. Three groups of improved native breeds, obtained from mating of ♂KUB to ♀KUB (Line 1), of ♂SenSi to ♀KUB (Line 2) and of ♂Gaok x ♀KUB (Line 3), were subjected to three experimental diets, differed in metabolizable energy (ME) and crude protein (CP) content. The experimental diets consisted of Diet 1 (2,800 kcal ME/ kg with 17,81% CP), Diet 2 (2,950 kcal ME/ kg with 18,61% CP) and Diet 3 (3,100 kcal ME/ kg with 19,25% CP). The experiment was designed as factorial 3 x 3 with 7 replications of each treatment combination, consisted of 5 birds per treatment combination. The chickens were raised up to 10 weeks of age. Results of the experiment showed that Line 2 (♂SenSi mated to ♀KUB) had highest body weight at 10 weeks of age (P<0.05), lowest feed conversion ratio (FCR) and highest European Production Efficiency Factor (EPEF), compared to other two lines. The appropriate diet for Line 2 was Diet 2. It could be concluded the crossbred line that was resulted from crossing of ♂SenSi to ♀KUB (Line 2), had potential to be used as improved native chicken for the industry in Indonesia supported by appropriate diet containing 2,950 kcal ME/kg with 18.61% crude protein.

This study was to determine the fruit maturity level of wild bitter gourd and the composition of water extract which is effective as antifeedant and appropriate for mortality test on armyworm larvae. Results obtained show that water extract of wild bitter gourd fruit contains phenolic compounds, flavonoids and triterpenoids. Terpenoid compounds contained in water extract of fruits at maturity level 4H, 8H and 12H were momordicoside L, momordicoside K, compound 3β, β7, 25-trihydroxycucurbita-5.23(E)-diena-19-al:R1=H, R2= H and momordicine 1. Fruit maturity level 4H 50% and 4H 60% resulted to the highest antifeedant index, ie 40.08% and 44.20%. LC 50 at fruit maturity level 4H observed on day 7 was 40%.

Antibiotics (AGP) have been used as feed additives to promote growth and feed efficiency in poultry production. However, many countries include Indonesia now ban the use of AGP and attempts are made to replace the antibiotic to maintain good performances of broilers. Plant bioactives is one of the alternatives that could replace the AGP. An experiment was conducted in an attempt to replace the AGP in broiler feed with a mixture of some plant bioactives<strong> </strong>(liquid smoke of cashew nutshell, <em>Phyllanthus niruri,</em> and clove leaves). Eight (8) dietary treatments were formulated to have similar nutrients consist of negative control (NC), positive control (NC+AGP), diets supplemented with liquid plant bioactives in 3 levels and diets supplemented with powder plant bioactives in 3 levels.  Each diet was fed to 6 replications of 10 birds each from 1 to 35 days old. The performances and the immune response of the broilers due to the treatments were observed. Results showed that the powder plant bioactives could not improve the performance of broilers. None of the feed additives (AGP or plant bioactives) affect the immune systems of the broilers. However,  liquid plant bioactives in low dose improved the performance of broilers better than the AGP and therefore is suitable to replace the antibiotic as feed additives in broiler diet.

Enzyme has been used widely in livestock industry in order to improve efficiency on feed utilization (FCR). Indonesian Research Institute for Animal Production has been able to produce enzyme BS4 which has been proven can improve nutrient digestibilities of some feedstuffs and the FCR on improved breed chickens. A research was designed to evaluate the effectivity of the BS4 enzyme on the growing local-KUB chickens. Three dietary treatments, i.e., control diet (C); C + low level BS4; and C + high level BS4 enzyme were fed to KUB chickens from 1 day old to 12 weeks old. Each dietary treatment was fed to 5 replicates x 24 birds per replicate reared on litter cages. Observations were made on body weight at 1 day, 3, 6, 9 and 12 weeks of age, feed intake, FCR and the mortality during the experiment. All data were analysed statistically in order to show the effect of the treatments. Results showed that BS4 enzyme supplementation did not show significant effect on mortality, feed intake and body weight of KUB chickens. However, supplementation of BS4 enzyme improved the FCR overall period of experiment, although it was only statistically significant (P<0.05) for the period of 0-9 weeks of age. It is concluded that supplementation of BS4 enzyme with dose 24 U/kg feed was effective to improve the FCR of the local-KUB chickens.