1) a standardized manufactured liquid formula was designedI0J containing components that were shown to challenge pp TG metabolism: saturated fat, sucrose, and ethanol;I2-l6 (2) this oral metabolic tolerance test (oMTT) was applied to a homogeneous group of 113 healthy male volunteers of similar age (25.0 f 0.3 years) and body mass index (BMI = 22.4 f 0.4 kg/m2)11 to avoid any variance in TG response by these ~ariables.5-'7-~9 0 This work was supported by the Stifterverband f i r die Deutsche Wissenschaft by the award of an H.-and L.-Schilling-Professorship to J. Schrezenmeir, and by the Institut Danone fir Erniihrung.353

A reliable simple reversed-phase liquid chromatographic method for the routine determination of ascorbic acid in plasma and urine with ultraviolet detection is described. This method enables the complete separation of the ascorbic acid peak from others with a recovery of above 95% within 8 minutes. The method can be used for analysing multiple samples within a day. In addition, the storage conditions and stability of ascorbic acid in plasma and urine were investigated. Samples of plasma and urine can be stored on ice in darkness for at least 60 min without reduction of ascorbic acid concentration. Prepared samples can be stored in darkness at 4 degrees C for at least 120 min and in liquid nitrogen for 42 days.

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In order to study the clinical effects of the antioxidant alpha-tocopherol (tocofersolan, CAS 30999-06-5) a simple, robust, sensitive and reliable high performance liquid chromatography (HPLC) method using fluorescence detection for the daily measurement of alpha-tocopherol concentration in plasma is described. Several methods of HPLC analysis using fluorescence techniques are published, however, investigations of the stability of alpha-tocopherol during sample preparation under different conditions are relevant in the routine measurements and have to date not been reported. Therefore a modified method, both sensitive and robust, qualified for the day-by-day measurement of alpha-tocopherol in plasma was established and the stability of alpha-tocopherol during sample preparation was analysed under different conditions. This method enables the complete separation of alpha-tocopherol from other compounds within 14 min. In addition, investigations on the stability of alpha-tocopherol during sample preparation showed the necessity of rapid sample preparation without delay. Prepared samples can be stored in liquid nitrogen for at least 56 days.