Introduction: The present study aimed to establish the staining protocol for quantification of elastic fibers and muscle fibers in arterial vessels through the Verhoeff method adapted with eosin in Chelonia mydas. Materials and Methods: Aorta and pulmonary arteries of 11 individuals of the species Chelonia mydas were used. The fragments were fixed in formaldehyde solution buffered 10% for 24 hours, subjected to routine histological processing and staining technique to be adapted Verhoeff, take the photografies and analyzed by Image Pro Plus Software. Results: The combination of ferric hematoxylin Verhoeff use blushed black elastic arteries blades, already eosin stained muscle fibers and collagen, allowing the tissue quantification through distinction staining by software. Conclusion: The protocol is a low-cost alternative that facilitate the acquisition of morphometric data for research with turtles.

The present study aimed to evaluate the insecticidal activity of hydroalcoholic plant extracts on Aphis craccivora Koch nymphs in cowpea. The experiments were carried out under greenhouse conditions in a randomized block design with five repetitions. Hydrated ethanol was used as a solvent in the botanical extract preparation. Cowpea plants were infested with five female adult aphids, eleven days after planting. After 48 hours, the adults were removed from the plants, leaving the recently bred nymphs. The evaluation of the nymphs’ survival was carried out forty-eight 48 hours after the application of the plant extracts. The botanical extracts with more than 50% efficiency were: Allium tuberosum leaf, Caesalpinia ferrea leaf, Piper aduncum leaf, Carica papaya seed, Dieffenbachia picta leaf, Cucurbita moschata seed and the control treatment, Annona squamosa seed. The botanical extracts with less than 50% efficiency were: Allium sativum bulb, Anacardium occidentale leaf, Borreria verticillata leaf plus flower, Coffea arabica leaf and seed, Delonix regia leaf and flower, Euphorbia tirucalli leaf, Leucaena leucocephala leaf, Mimosa caesalpiniaefolia leaf, Nerium oleander leaf, Syzygium cumini leaf, endocarp and pericarp, Syzygium malaccense leaf, Zingiber officinale rizome and Ziziphus joazeiro leaf. The highest efficiency levels were obtained by D. picta and C. moschata, rendering these extracts suitable for field trials to further evaluate their efficieny against the cowpea black aphid.

In regions with high temperatures, carrot cultivation is impracticable, as high temperatures tend to reduce yield and quality of the product. However, with the advent of summer cultivars, carrot cultivation in these regions has become viable. The aim of this work was to evaluate the performance of ten carrot cultivars in different planting seasons. The experiments were carried out at the Rafael Fernandes Experimental Farm of the Federal Rural Semiarid University (UFERSA). The experiments were carried out in May, June, July and August of 2017. The experimental design was a randomized block with ten treatments and four repetitions. The characteristics that were evaluated were the root classification (long, average and short roots); commercial, non-commercial and total yield; dry plant weight and root fresh weight. Both the planting season and the cultivar played important roles in agronomic performance throughout the experimental period. In May and June, the highest average yields and plant growth were obtained (43 and 45 t ha-1 yield, respectively). Hybrid cultivars showed better performance than open-pollinated cultivars in terms of yield. The Brasília, BRS Planalto, and Kuronan varieties showed poor performance in all the evaluated characteristics.

Micronutrients structurally constitute several enzymes and act as a cofactor of essential proteins to maintain cell function, thereby contributing to crop growth and yield. The objective of this study was to evaluate the leaf content, growth, accumulation of micronutrients, classification and yield of onion as a function of fertilization with boron, cooper and zinc in two years of cultivation. The experiments were carried out from June to November in 2018 and 2019, in a soil classified as Ultisol, both at the Rafael Fernandes Experimental Farm, belonging to the Universidade Federal Rural do Semiárido, in the municipality of Mossoró, Rio Grande do Norte, Brazil. The experimental design was in randomized blocks with 15 treatments and four replicates. The treatments consisted of application of doses of B, Cu and Zn, in two experiments. Contents of B, Cu and Zn in the diagnostic leaf, growth, accumulation of B, Cu and Zn in the leaf, bulb and total, classification and commercial, non-commercial and total yields were evaluated. Application of B, Cu and Zn did not influence the number of leaves, relation of bulb shape, leaf, bulb, and total dry mass and yield of onion. Application of B, Cu and Zn, respectively at doses of 1-2-1 kg ha-1 favored a greater accumulation of B, Zn and Cu in the bulb. Higher number of leaves, leaf dry mass, bulb dry mass, total dry mass, class 1 bulbs and non-commercial yield were produced in Experiment 1.

The objective of this study was to evaluate the efficiency of the use of phosphorus by maize cultivars for the production of green ears. Two experiments were carried out, one in the rainy season (March to June) and the other in the dry period (August to December), both at the Rafael Fernandes Experimental Farm, in the community of Alagoinha, belonging to the Universidade Federal Rural do Semiárido, in the municipality of Mossoró, RN, Brazil. The treatments consisted of a combination of three levels of phosphorus (low, medium and high) and eight corn cultivars (Dona Josélia, Carrapateira, Pontinha, Cruzeta, Potiguar, BRS 2022, Status and AG 1051). A randomized block design was used in a 3 × 8 factorial scheme, with four repetitions. The evaluated characteristics were: number and total mass of green ears, number and mass of tradable, stuffed and depleted green ears, agronomic and physiological efficiency of production of recovery and use of phosphorus. The cultivars showed better productive performance when fertilized with phosphorus. The cultivars Cruzeta, Potiguar, BRS 2022, Status, AG 1051 and Carrapateira were the most efficient in the use of phosphorus to produce green maize.