Background.CD177, an indicator of prognosis in diverse cancers, is involved in the physiological processes of various tumor cells, and acts as an immune molecule with novel functions in cancer pathogenesis. However, the diagnostic, prognostic and immunological role of CD177 in cervical cancer remains unclear. Methods.Utilizing publicly available databases and integrating several bioinformatics analysis methods, we evaluated the expression level of CD177 in cervical cancer by GENT2, HPA and GEO databases. The Kaplan-Meier Plotter database, Xena Shiny and the constructed of a nomogram were clearly demonstrated its prognostic value for patients. Gene set enrichment analysis explored the relationship between CD177 and cervical cancer immune responses and immune cells in ltration level. In addition, we investigated the association between CD177 expression and stromalscore, immunescore, immune checkpoint and drug sensitivity by TCGA RNA-seq data. Results.CD177 was apparently expressed at low levels in cervical cancer and predicted a poor survival rate for patients. CD177 signi cantly activated immune-related signaling pathways and had a positive relationship with immune cell in ltration level. The high CD177 expression group possessed the high stromalscore and immunescore. CD177 had potential interactions with CTLA4, CD27, BLTA, CD200R1, CD80, NRP1, TNFRSF25, TIGIT, ICOS and TNFSF9 checkpoint markers. And CD177 expression was positively relevant with drug sensitivity for Lapatinib, Belinostat, ATRA, Ge tinib, Navitoclax and Tamoxifen. Signi cance.These ndings may shed light on the vital role of CD177 in cervical cancer diagnosis, prognosis, and immunological function, and it may be a promising predictor and potential factor for cervical cancer patients.

The anatomical positions of pelvic floor organs are maintained by ligaments and muscles. Stress urinary incontinence (SUI) occurs when the pelvic floor tissues are repeatedly stimulated by excessive mechanical tension that exceeds the bearing capacity of ligaments or muscles. Besides, cells respond mechanically to mechanical stimulation by reconstituting the Piezo1 and cytoskeletal system. The aim of this study is to determine how Piezo1 and actin cytoskeleton are involved in the mechanized stretch (MS) induced apoptosis of human anterior vaginal wall fibroblasts (hAVWFs) and the mechanism. A four-point bending device was used to provide mechanical stretching to establish a cellular mechanical damage model. The apoptosis of hAVWFs cells in non-SUI patients was significantly increased by MS, which exhibited apoptosis rates comparable to those of SUI patients. Based on these findings, Piezo1 connects the actin cytoskeleton to the apoptosis of hAVWFs cells, providing an idea for the clinical diagnosis and treatment of SUI. However, the disassembly of the actin cytoskeleton suppressed the protective effect of Piezo1 silencing on MS. Based on these findings, Piezo1 connects the actin cytoskeleton to apoptosis of hAVWFs, providing new insight for the clinical diagnosis and treatment of SUI.

Ligaments and muscles maintain the anatomical positions of pelvic floor organs. Stress urinary incontinence (SUI) occurs when pelvic floor tissues are repeatedly stimulated with excessive mechanical tension that is beyond the capacity of ligaments or muscles to endure. In addition, cells respond mechanically to mechanical stimulation by reconstituting the Piezo1 and cytoskeletal system. This study sought to determine how Piezo1 and actin cytoskeletal contribute to MS-induced apoptosis in human anterior vaginal wall fibroblasts (hAVWFs) and the mechanisms involved. A four-point bending device was used to provide mechanical stretching to establish a cellular mechanical damage model . MS significantly induced apoptosis of hAVWFs from non-SUI patients, which exhibited apoptosis rates comparable to those of SUI patients, and silencing of Piezo1 decreased the MS-induced apoptosis. However, the disassembly of actin cytoskeleton suppressed the protective effect of Piezo1 silencing from MS. Based on these findings, Piezo1 links the actin cytoskeleton to apoptosis in hAVWFs, providing insight into the clinical diagnosis and treatment of SUI.

Background. CD177, an indicator of prognosis in diverse cancers, is involved in the physiological processes of various tumor cells, and acts as an immune molecule with novel functions in cancer pathogenesis. However, the diagnostic, prognostic and immunological role of CD177 in cervical cancer remains unclear. Methods. Utilizing publicly available databases and integrating several bioinformatics analysis methods, we evaluated the expression level of CD177 in cervical cancer by GENT2, HPA and GEO databases. The Kaplan–Meier Plotter database, Xena Shiny and the constructed of a nomogram were clearly demonstrated its prognostic value for patients. Gene set enrichment analysis explored the relationship between CD177 and cervical cancer immune responses and immune cells infiltration level. In addition, we investigated the association between CD177 expression and stromalscore, immunescore, immune checkpoint and drug sensitivity by TCGA RNA-seq data. Results. CD177 was apparently expressed at low levels in cervical cancer and predicted a poor survival rate for patients. CD177 significantly activated immune-related signaling pathways and had a positive relationship with immune cell infiltration level. The high CD177 expression group possessed the high stromalscore and immunescore. CD177 had potential interactions with CTLA4, CD27, BLTA, CD200R1, CD80, NRP1, TNFRSF25, TIGIT, ICOS and TNFSF9 checkpoint markers. And CD177 expression was positively relevant with drug sensitivity for Lapatinib, Belinostat, ATRA, Gefitinib, Navitoclax and Tamoxifen. Significance. These findings may shed light on the vital role of CD177 in cervical cancer diagnosis, prognosis, and immunological function, and it may be a promising predictor and potential factor for cervical cancer patients.