With the implementation of the regional layout plan for advantageous agricultural products of the Ministry of Agriculture, the circulation of fruits and vegetables had the characteristics of elongated transportation distance, off-season, and cross-regional, which put forward higher requirements for the storage and preservation of fruits and vegetables. At present, fruit and vegetable storage technology had developed vigorously. The primary breakthroughs had been made in low temperature, controlled atmosphere storage, and other technologies. Some fruits had basically reached the annual supply (Qiao, 2014).However, every year in China, 15-20% of fresh fruits and vegetables still lose their commodity value due to quality deterioration and disease rot. The economic losses exceed 100 billion yuan.

No abstract

Background: It was demonstrated in our previous research that trypsin scavenges superoxide anions. In this study, the mechanisms of storage quality improvement by trypsin were evaluated in H. undatus. Results: Trypsin significantly delayed the weight loss and decreased the levels of ROS and membrane lipid peroxidation. Transcriptome profiles of H. undatus treated with trypsin revealed the pathways and regulatory mechanisms of ROS genes that were up- or downregulated following trypsin treatment by gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes pathway (KEGG) enrichment analyses. The current results showed that through the regulation of the expression of hub redox enzymes, especially thioredoxin-related proteins, trypsin can maintain low levels of endogenous active oxygen species, reduce malondialdehyde content and delay fruit aging. In addition, the results of protein-protein interaction networks suggested that the downregulated NAD(P)H and lignin pathways might be the key regulatory mechanisms governed by trypsin.Conclusions: Trypsin significantly prolonged the storage life of H. undatus through regulatory on the endogenous ROS metabolism. As a new biopreservative, trypsin is highly efficient, safe and economical. Therefore, trypsin possesses technical feasibility for the quality control of fruit storage.

No abstract

No abstract