2013) Characterisation, genetic diversity and antagonistic potential of 2,4-diacetylphloroglucinol producing Pseudomonas fluorescens isolates in groundnut-based cropping systems This study is focused on isolation and characterisation of 2,4-diacetylphloroglucinol (2,4-DAPG)-producing Pseudomonas fluorescens isolates from different soils of groundnut-based cropping systems in Andhra Pradesh. In our studies, 21 isolates of P. fluorescens were isolated and confirmed through various biochemical tests, of which five were tested positive for 2,4-DAPGproduction with specific primers. Biocontrol potential of these isolates on groundnut stem rot pathogen (Sclerotium rolfsii) was determined through in vitro dual culture assays. The eight isolates were found effective against S. rolfsii (up to 75% inhibition) in dual culture method. All the five 2,4-DAPG-producing Plant Growth-Promoting Rhizobacteria isolates were highly antagonistic to S. rolfsii. Genetic diversity of these P. fluorescens isolates was determined by random amplification of polymorphic DNA analysis. Overall, our results suggest that the prevalence of 2,4-DAPG-producing fluorescent Pseudomonads in different crop rhizospheres of groundnut-based cropping systems.

The natural occurrence of Groundnut Bud Necrosis Virus (GBNV) on Colocasia esculenta was detected by enzyme linked immunosorbent assay using an antiserum raised against GBNV and reverse transcription polymerase chain reaction using coat protein specific primers. Sequence analysis showed 93-99% and 95-99% identity at nucleotide and amino acid levels respectively with other reported GBNV isolates.

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The natural occurrence of Groundnut bud necrosis virus (GBNV) on onion (Allium cepa L.) was detected by enzyme linked immunosorbent assay using an antiserum raised against GBNV and reverse transcription polymerase chain reaction using coat protein gene specific primers. Sequence analysis showed 93-100 % and 95-100 % identity at nucleotide and amino acid levels respectively with other reported GBNV isolates.