Background The high−fat, high−fructose diet (HFFD) provokes overnutrition and inflammation directly, mainly through Toll−like receptors (TLRs). Soybean ( Glycine max L.) contains isoflavone that can be transformed into glyceollin by microbial and physical stimuli. Glyceollin possesses many beneficial effects on health. Objective This study evaluates the beneficial effect of soybean extract elicited by Saccharomyces cerevisiae and light (ESE) on dendritic cells (DCs) profile and naïve T cells in HFFD mice. Materials and methods Female Balb/C mice were fed with HFFD for 24 weeks then orally administered with simvastatin 2.8 mg/kg BW or ESE 78, 104, and 130 mg/kg BW at the last four weeks. The expression of splenic CD11c + TLR3 + , CD11c + TLR4 + , NFκB + , CD11c + IL-17 + , CD11c + TNF−α + , CD4 + CD62L + , and CD8 + CD62L + subsets was measured by flow cytometry. The molecular docking has been measured using Pyrx 0.8, displayed in PyMol and Biovia Discovery Studio. Result HFFD significantly increased CD11c + TLR3 + , CD11c + TLR4 + , NFκB + , CD11c + IL-17 + , CD11c + TNF−α + expression and decreased CD4 + CD62L + and CD8 + CD62L + ( p < 0.05) compared to normal diet (ND) groups. ESE reduced CD11c + TLR3 + , CD11c + TLR4 + , thereby decreasing NFκB + , as well as decreased the CD11c + IL−17 + , CD11c + TNF−α + , and restores CD4 + CD62L + and CD8 + CD62L + subsets in HFFD mice. Glyceollin II exhibited the best binding affinity with an average energy of −7.3 kcal/mol to TLR3 and −7.9 kcal/mol to TLR4. Conclusion The bioactive compound in ESE act synergistically to modulate TLR3/TLR4 activation, reduced NFκB, IL−17, and TNF−α, and restores naïve T cells expression in HFFD mice. ESE was a favorable candidate to mitigate chronic inflammation.

Introduction: The relationship between B cells and Toll-like Receptors (TLRs) under high fat−high fructose diet (HFFD) is still poorly understood. Isoflavone content from soybean can be modified by using biotic and abiotic elicitors to synthesize glyceollin. Glyceollin has gained much interest in recent past year due to its beneficial effects on health. The study aimed to examine the ameliorative effect of elicited soybean extract (ESE) on B cells profile in HFFD mice. Material and Methods: Twenty-eight female Balb/C mice were divided into normal diet (ND), ND + ESE 104 mg/kg, HFFD, HFFD + Simvastatin 2.8 mg/kg, ESE 78 mg/kg, 104 mg/kg, and 130 mg/kg, respectively. Mice were fed with HFFD for 24 weeks and ESE was administered orally per day at last 4 weeks. At week 24, the animals were sacrificed and the spleen was collected. B cells were labeled as B220 + TLR3 + , B220 + TLR4 + , B220 + NFκB-, and B220 + NFκB + and the B cells expression were measured by flow cytometry. Molecular modeling was performed by Pyrx 0.8 and visualized in PyMol. Results: The ESE treatment significantly decreased B220 + TLR3 + , B220 + TLR4 + , and B220 + NFκB + expression and restored B220 + NFκB − expression in HFFD mice (p<0.05). Glyceollin I exerted the lowest binding affinity with estimated energy was-7.0 kcal/mol at NFκB. Conclusions: ESE administration ameliorates HFFD-induced inflammation by modulating TLR3/TLR4 activation and prevents NFκB expression of B cells in HFFD mice. ESE exerts as a promising agent in the future and provides a better understanding mechanism to treat chronic inflammation caused by HFFD.

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The East Java Religious High Court survey shows that the rate of early marriage in Bondowoso is the highest in Indonesia. In fact, BKKBN recommends the age of marriage for women 21 years and men 25 years. The recommendation is based on several factors such as health factors and psychological factors. In addition, the risk of divorce is also part of the risk of early marriage, which at that age teenagers are more likely to seek identity so that at that time they are emotionally unstable. However, not all early marriages end in divorce. Therefore, the researcher wants to know about what factors affect the harmony of the household of early marriage couples. This research was conducted in Alassumur village, Kec. Pujer Bondowoso. Based on the observations of researchers, the village government of Alassumur took part in efforts to minimize early couple household conflicts in the village. This research is a qualitative descriptive study. By conducting in-depth interviews for data collection. The results of this study indicate that the role of opinion leaders has a strong influence on the village community.

Demam berdarah merupakan salah satu kasus penyakit tropis yang masih sering ditemui di berbagai wilayah di Indonesia. Pencegahan yang bisa dilakukan masyarakat untuk terhindar dari penyakit demam berdarah adalah dengan pemakaian anti nyamuk, tetapi anti nyamuk yang banyak digunakan masyarakat saat ini diketahui menggunakan bahan kimia N,N-diethyl-3-methylbenzamide (DEET) yang juga memiliki efek negatif ke kulit yang sensitif. Pemanfaatan bahan alam bisa dijadikan solusi pengganti lotion anti nyamuk yang lebih ramah lingkungan dan aman untuk kulit yang sensitif. Berdasarkan penelitian yang ada, tanaman serai memiliki kandungan minyak serai yang efektif untuk mengusir serangga, salah satunya adalah nyamuk. Kadungan utama minyak serai adalah citronellal, citronellol dan geraniol yang diketahui memiliki efektifitas sebagai penolak nyamuk. Kegiatan pengabdian masyarakat ini bertujuan untuk memberikan edukasi tentang bahaya demam berdarah serta memberdayakan masyarakat sekitar untuk mengolah tanaman serai menjadi lotion anti nyamuk yang aman dan murah. Kegiatan pengabdian masyarakat ini dilaksanakan pada bulan Desember 2021 sampai dengan Januari 2022. Metode yang dilakukan yakni dengan pemberian pre-test dan post-test terkait pengetahuan masyarakat terhadap demam berdarah, dilanjutkan sosialisasi, edukasi serta pembuatan lotion anti nyamuk yang dibimbing langsung oleh pengabdi. Hasil pengabdian ini menunjukkan tercapainya peningkatan pengetahuan masyarakat tentang bahaya demam berdarah dari 48% menjadi 85%, serta keberhasilan masyarakat untuk membuat lotion anti nyamuk dari batang serai secara mandiri. Dengue fever is a tropical disease still often found in some regions in Indonesia. Dengue prevention can be done by using mosquito repellents, but the mosquito repellents widely used today are known to use the chemical N, N-diethyl-3-methylbenzamide (DEET), which also harms sensitive skin. Some natural ingredients are known to have the ability to repel mosquitoes; one of them is lemon grass. Based on existing research, the lemongrass plant contains lemongrass oil which is effective for repelling insects, such as mosquitoes. The main ingredients of citronella oil are citronellol, citronellol and geraniol, which are known to have effectiveness as a mosquito repellent. This community service series aims to educate about the dangers of dengue fever and empower the surrounding community to process lemongrass plants into mosquito repellent lotions that are safe and inexpensive. This community service activity is carried out from December 2021 to January 2022. The method used is by giving pre-test and post-tests related to public knowledge about dengue fever, followed by socialization, education and making mosquito repellent guided directly by the service. The results of this service show the achievement of increasing public knowledge about the dangers of dengue fever from 48% to 85%, as well as the community's success in making mosquito repellent lotions from lemongrass stems independently.