No abstract

Recently, some metabolites in skin interstitial fluid (SIF) have become emerging re×sources for early disease diagnosis. However, their low level in SIF and difficulty to sampling are the biggest obstacle to further potential application. Here, a swellable microneedle array patch (MNAP) with high mechanical strength is presented, and the rapid enrichment of positively charged metabolites is achieved. The MNAP is fabricated by poly (chondroitin sulfate-acrylamido-2-methylpropane sulfonic acid)-gold nanoparticles (GNPs) composites via a micro-molding. The negatively charged copolymer hydrogel not only enrich positively charged metabolites, but also provide swellable capacity. The in situ synthesis of GNPs in the process of copolymerization make the GNPs cross-link to the hydrogel, which further enhance the MNAP mechanical strength and enrichment efficiency for positively charged metabolites. By using the MNAP, around 5 mg SIF in 10 min from the high fat/cholecalciferol/methimazole-induced atherogenesis rat is extracted and 23 metabolites including 13 quaternary ammonium cationic compounds can be detected and quantified by using a LC-QTOF-MS. Dysregulated L-carnitine and choline metabolism are discovered a week earlier in the SIF than in the serum, achieving early diagnosis of the metabolism syndrome disease. This MNAP also helps users complete home sampling for early disease diagnosis and monitoring.

No abstract

Multi-functional and hierarchically structured silica nanospheres are rationally designed and fabricated, which encapsulate quantum dots to permit near infrared deep tissue imaging and are loaded with carcinoembryonic antigen messenger RNA (CEAmRNA) to enable stable and abundant antigen expression in DCs.

Mediating mitochondria function is essentially challenging but actually significance for biomedicine. However, remote non-invasive regulation of mitochondria function in a living system has been seldom demonstrated and further developed for potential therapy. In here, we prepared a seleno-polysaccharide polymer nanocomposite owning photothermal activity to increase anti-tumor therapy capacity. Such a nanocomposite comprises two key constitutions: near infrared polymer amphiphile and seleno-polysaccharide, acting as photothermal nanotransducer and temperature-sensitive reactive oxygen species (ROS) accelerator, respectively. Under near infrared irradiation (NIR), the productivity of intracellular reactive oxygen species (ROS) can be increased by 1.5-fold to efficiently mitochondrial dysfunction, leading to endogenous mitochondrial apoptosis. This design thus enables provide a promising approach to remotely mediate mitochondria-mediated apoptosis for anti-tumor therapy.