Purpose: To employ high performance liquid chromatography (HPLC)

No abstract

In this paper, the thermoelectric, spin-dependent optical and quantum transport properties of two-dimensional(2D) Co2Se3 monolayer are investigated using first principle calculations. The stability of Co2Se3 monolayer is confirmed by energy-cohesive...

NOR loci of C-subgenome are dominant in wheat × Aegilops interspecific hybrids, which may have evolutionary implications for wheat group genome dynamics and evolution. After interspecific hybridisation, some genes are often expressed from only one of the progenitor species, shaping subsequent allopolyploid genome evolution processes. A well-known example is nucleolar dominance, i.e. the formation of cell nucleoli from chromosomes of only one parental species. We studied nucleolar organizing regions (NORs) in diploid Aegilops markgrafii (syn: Ae. caudata; CC), Ae. umbellulata (UU), allotetraploids Aegilops cylindrica (CCDD) and Ae. triuncialis (CCUU), synthetic interspecific F hybrids between these two allotetraploids and bread wheat (Triticum aestivum, AABBDD) and in F generation hybrids with genome composition AABBDDCCUU using silver staining and fluorescence in situ hybridization (FISH). In Ae. markgrafii (CC), NORs of both 1C and 5C or only 5C chromosome pairs were active in different individual cells, while only NORs on 1U chromosomes were active in Ae. umbellulata (UU). Although all 35S rDNA loci of the C subgenome (located on 1C and 5C) were active in Ae. triuncialis, only one pair (occupying either 1C or 5C) was active in Ae. cylindrica, depending on the genotype studied. These C-genome expression patterns were transmitted to the F and F generations. Wheat chromosome NOR activity was variable in Ae. triuncialis × T. aestivum F seeds, but silenced by the F generation. No effect of maternal or paternal cross direction was observed. These results indicate that C-subgenome NOR loci are dominant in wheat × Aegilops interspecific hybrids, which may have evolutionary implications for wheat group genome dynamics and allopolyploid evolution.

In this project, a simple, low-cost and rapid procedure based on dispersive liquid-liquid microextraction (DLLME) technique coupled with high performance liquid chromatography-ultraviolet detector (HPLC-UV) has been used for the extraction and determination of styrene in aqueous solutions. Several factors, such as type of extraction and dispersive solvents and their volumes, salt addition, and pH were optimized. Under optimal conditions, the recoveries of styrene for tea and water samples spiked with 10 and 15 ng mL −1 were in the range of 91.4-97.8 %, whereas the temperature was set at 0, 4, 20, 70 and 91°C for 15, 30, 60, 1440, and 14,400 min. The linear range was obtained in the interval of 1.86-50 ng mL −1 . The limits of detection (S/N = 3) and quantitation (S/N = 10) were 0.6 and 1.86 ng mL −1 , respectively. The relative standard deviations (RSDs) for three replicated analysis of styrene in aqueous samples ranged from 0.01 to 0.3 %.