Expression of XbP1 in lymphocytes of

The objective of the present work was to study the morphofunctional state of insulin-expressing, antigen-presenting, Treg cells, epithelioreticulocytes, thymic cells expressing c-Fos and Bcl-2+ in the progeny of rats with experimental gestational diabetes (EGD). The methods employed included immunohistochemical staining with direct and indirect immunofluorescence using monoclonal antibodies against insulin, MHC-II antigen, CD25 antigen, cytokeratins, rat Bcl-2 and c-Fos proteins. The off-springs of rats with experimental gestational diabetes were shown to have multiple disturbances of immunoregulation in the thymus, such as abnormalities of Treg cell differentiation, impaired expression of early response proteins (c-Fos), reduced number of epithelioreticulocytes, antigen-presenting and insulin-immunopositive cells, hyperexpression of anti-apoptotic Bcl-2 protein. These changes interfere with the normal apoptotic process, selection of thymocytes, and development of central tolerance to pancreatic antigens.