Actuality. Pre-eclampsia during pregnancy is a major cause of maternal mortality, intra- and neonatal pathology [1]. Sharply there is a question about the search method of determining the predictors of pre-eclampsia and the degree of influence on a woman’s body for the purpose of timely diagnosis and treatment. Interest in the method of determining the parameters of arterial stiffness is growing every year because of the availability and relative ease of use to determine the degree of maladjustment of the vascular system and assessing the risks of cardiovascular disease.Objective. Evaluation of indicators characterizing stiffness of the arterial wall in pre-eclampsia during pregnancy and after childbirth.Materials and Methods. In a prospective cohort comparative study included 62 pregnant women aged 19 to 35: 35 women with physiological pregnancy without physical abnormalities and 27 women with preeclampsia moderate, without preeclampsia history. Surveys were carried out in time for 22-24, 32-34 weeks of gestation and at 8 hours after delivery. To evaluate the rigidity of the vascular wall used complex BPLab.Results. In pregnant women with pre-eclampsia there is an increase of arterial stiffness parameters at 22 weeks of pregnancy in comparison with the original data at 12 weeks of gestation, IA uvelichilsyana 29.3%, 12.5% PWV. Most significantly in the intervention group to the third trimester have changed the following parameters: IA averaged – - 28% (± 5,5) + 37% from baseline, PWV – 8 m/s (± 0,9) + 12,5%, in the control group: IA – - 39,6% (± 8,1) + 12,2% in comparison with the data of 12 weeks’ gestation, PWV – 7.43 m/s (± 0,82) + 12,5%, demonstrating various adaptive features of the vascular wall in groups. In the early postpartum period significantly positive dynamics of arterial stiffness parameters in the group with physiological pregnancy, pre-eclampsia in the group with the values remain the same or change slightly. Conclusions. Thus, from the data of the pulse wave analysis is the best method for measuring vascular stiffness non-invasively in pregnant women. This method demonstrates the physiological adaptation of vessels to the gestational process, and shows the changes associated with preeclampsia as the background of pregnancy and after childbirth. Thus, this method makes it possible to evaluate the adaptive reserve of the vascular wall for the purpose of forecasting the development of cardiovascular complications.

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Aim. To study the nature of CYP24A1 gene expression in the placental tissue in women with different saturation of the body with vitamin D during normal pregnancy and preeclampsia.Materials and methods. Cohort retrospective and prospective study. There were examined 110 pregnant women in Saint Petersburg and Leningrad region, who took multivitamin complex containing 400 IU of vitamin D from 12 weeks of pregnancy. Time of inclusion is from September to June. All pregnant women were taken blood samples with the following determination of the level of 25-hydroxycalciferol, fragments of placental tissue with the following study of mRNA expression of CYP24A1 gene.Results. There were analyzed two groups of pregnant women. During the analysis of the saturation level of the body with vitamin D there were revealed the following things: the frequency of vitamin D deficiency in the main group was 69.6% in the group of comparison vitamin D deficiency was not found. The frequency of vitamin D insufficiency in the main group was 21.5% in the group of comparison – 18.5%. The frequency of the normal level of vitamin D in the main group was 8.9% in the group of comparison – 81.5%. Differences between the main group and the group of comparison are reliable (p < 0,0001). There revealed a statistically significant increase of the relative expression of mRNA of CYP24A1 gene in the placental tissue in women with preeclampsia (31,8 ± 20,6), in comparison with women with physiological pregnancy (11,2 ± 17,9) (p = 0.03)Conclusion. In women with preeclampsia low level of vitamin D in blood serum (less than 30 ng/ml) is 5 times more common, that is why insufficiency and deficiency of vitamin D in pregnant women can be attributed to risk factors for the development of preeclampsia. Expression of mRNA of CYP24A1 gene in placental tissue in patients with preeclampsia is three times higher in comparison with patients with a normal pregnancy. Thus, the increased expression of CYP24A1 gene in the placental tissue can be considered as one of the risk factors for the development of preeclampsia.