A label free nanosensing platform for the detection of cervical cancer through analysis of ultratrace DNA hybridization

Pareek, Sakshi; Jain, Utkarsh; Bharadwaj, Mausumi; Chauhan, Nidhi

doi:10.1016/j.sbsr.2021.100444

Cited by 12 publications

(9 citation statements)

References 28 publications

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“…Since no label is attached to the molecules, therefore, the actual nature and data of the biodegradable material remain constant. Furthermore, it is possible to use the biosensors in cancer research for analyzing the changes in proteins or target cell lines [51]. Therefore, all-optical biosensors have been recognized to be proper for disposable rapid detection.…”

Section: Introductionmentioning

confidence: 99%

RETRACTED: Ultra-Sensitive Biosensor with Simultaneous Detection (of Cancer and Diabetes) and Analysis of Deformation Effects on Dielectric Rods in Optical Microstructure

et al. 2021

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This study proposes a refractive index sensor for the simultaneous detection of cancer and diabetes based on photonic crystals (PhC). The proposed PhC composed of silicon rods in the air bed arranged in a hexagonal lattice forms the fundamental structure. Two tubes are used to place the cancerous or diabetic samples for measurement. The sensor’s transmission characteristics are simulated and analyzed by solving Maxwell’s electromagnetic equations using the finite-difference time-domain approach for samples being studied. Therefore, diabetes and cancer are detected according to the changes in the refractive index of the samples using the laser source centered at 1550 nm. Considering the findings, the sensor’s geometry changes to adjust the suggested sensitivity and quality factor of structure. According to the results, transmission power ranges between 91 and 100% based on the sample. Moreover, sensitivity ranges from 1294 to 3080 nm/RIU and the maximum Figure of Mertie is nearly FOM = 1550.11 ± 150.11 RIU−1 with the detection in range 31 × 10−6 RIU. In addition, the small area (61.56 μm2) of biosensor results in its appropriateness for different uses in compact photonic integrated circuits. Next, we changed the shape of the dielectric rods and investigated their effects on the sensitivity parameter. The sensitivity and figure of merit after changes in the shape of dielectric rods and nanocavities are at best S = 20,393 nm/RIU and FOM = 9104.017 ± 606.93 RIU−1, receptively. In addition, the resolution detection range is 203.93 × 10−6 RIU.

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Section: Introductionmentioning

confidence: 99%

RETRACTED: Ultra-Sensitive Biosensor with Simultaneous Detection (of Cancer and Diabetes) and Analysis of Deformation Effects on Dielectric Rods in Optical Microstructure

et al. 2021

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“…The conventional strategies for detecting the presence of HPV-16 infections includes histological measurements and analysing cytological fluctuations using biopsy or Papanicolaou (Pap) screening methods [ 4 ]. The strategies involving the latter, hybrid capture assay and colposcopy, suffer from low sensitivity and specificity while being time consuming [ 5 ]. Meanwhile, polymerase chain reaction (PCR) has comparatively high sensitivity and is currently being widely employed for HPV detection, albeit with limitations of its own, such as the requirement of skilled manpower, high cost per test and tedious DNA extraction protocol [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

“…The strategies involving the latter, hybrid capture assay and colposcopy, suffer from low sensitivity and specificity while being time consuming [ 5 ]. Meanwhile, polymerase chain reaction (PCR) has comparatively high sensitivity and is currently being widely employed for HPV detection, albeit with limitations of its own, such as the requirement of skilled manpower, high cost per test and tedious DNA extraction protocol [ 5 ]. Furthermore, the presence of limited cyto technicians in every hospital or clinic in developing nations has detrimental effects on the affected patients, leading to delayed treatment with dreadful outcomes [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

“…It has also been observed that some interpretations of the cytology images are often vague, while being greatly prone to interference in the presence of blood and vaginal discharge leading to false positive or negative results [ 6 ]. It must be noted that the above screening tests are undertaken specifically after observing cervical cancer-related symptoms, whereby the situation may have worsened by the time patients begin with implementing the necessary treatment protocols [ 5 , 6 ]. Therefore, it becomes extremely important to develop highly sensitive, rapid and cost-effective biosensing platforms which can detect the occurrence of cervical cancer, by monitoring HPV 16 activity, at its onset.…”

Section: Introductionmentioning

confidence: 99%

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An Electroanalytical Flexible Biosensor Based on Reduced Graphene Oxide-DNA Hybrids for the Early Detection of Human Papillomavirus-16

et al. 2022

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Human Papilloma Virus 16 (HPV 16) is the well-known causative species responsible for triggering cervical cancer. When left undiagnosed and untreated, this disease leads to life-threatening events among the female populace, especially in developing nations where healthcare resources are already being stretched to their limits. Considering various drawbacks of conventional techniques for diagnosing this highly malignant cancer, it becomes imperative to develop miniaturized biosensing platforms which can aid in early detection of cervical cancer for enhanced patient outcomes. The current study reports on the development of an electrochemical biosensor based on reduced graphene oxide (rGO)/DNA hybrid modified flexible carbon screen-printed electrode (CSPE) for the detection of HPV 16. The carbon-coated SPEs were initially coated with rGO followed by probe DNA (PDNA) immobilization. The nanostructure characterization was performed using UV-Vis spectroscopy, Fourier transform infrared (FTIR) spectroscopy, Raman spectroscopy and X-ray diffraction (XRD) techniques. Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) were employed to study the electrochemical characterization of the nano-biohybrid sensor surface. The optimization studies and analytical performance were assessed using differential pulse voltammetry (DPV), eventually exhibiting a limit of detection (LoD) ~2 pM. The developed sensor was found to be selective solely to HPV 16 target DNA and exhibited a shelf life of 1 month. The performance of the developed flexible sensor further exhibited a promising response in spiked serum samples, which validates its application in future point-of-care scenarios.

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“…Today, most of the electrochemical methods of detecting HPV have been focused on nucleic acid (DNA) sensors. − To our knowledge, the use of protein biomarkers for the electrochemical detection of HPV is largely unknown. There are only a few reports that attempted the utilization of HPV protein biomarkers. ,− For example, Piro et al reported the use of a very complex conjugated electro-co-polymer to encapsulate the antigenic HPV-16 L1 on a glassy carbon electrode (GCE) and used it to detect just a single concentration of the anti-HPV-16 L1 antibody.…”

mentioning

confidence: 99%

Electrochemical Immunosensor for Ultra-Low Detection of Human Papillomavirus Biomarker for Cervical Cancer

et al. 2023

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Human papillomavirus (HPV) is the causative agent for cervical cancer. Of the various types of HPV, the high-risk HPV-16 type is the most important antigenic high-risk HPV. In this work, the antigenic HPV-16 L1 peptide was immobilized on a glassy carbon electrode and used to detect several concentrations of the anti-HPV-16 L1 antibody, and vice versa. Two electrode platforms were used: onion-like carbon (OLC) and its polyacrylonitrile (OLC-PAN) composites. Both platforms gave a wide linear concentration range (1.95 fg/mL to 6.25 ng/mL), excellent sensitivity (>5.2 μA/log ([HPV-16 L1, fg/mL]), and extra-ordinarily low limit of detection (LoD) of 1.83 fg/mL (32.7 aM) and 0.61 fg/mL (10.9 aM) for OLC-PAN and OLC-based immunosensors, respectively. OLC-PAN modified with the HPV-16 L1 protein showed low LoD for the HPV-16 L1 antibody (2.54 fg/mL, i.e., 45.36 aM), proving its potential use for screening purposes. The specificity of detection was proven with the anti-ovalbumin antibody (anti-OVA) and native ovalbumin protein (OVA). An immobilized antigenic HPV-16 L1 peptide showed insignificant interaction with anti-OVA in contrast with the excellent interaction with anti-HPV-16 L1 antibody, thus proving high specificity. The application of the immunosensor as a potential point-of-care (PoC) diagnostic device was investigated with screen-printed carbon electrodes, which detected ultra-low (ca. 0.7 fg/mL ≈ 12.5 aM) and high (ca. 12 μg/mL ≈ 0.21 μM) concentrations. This study represents the lowest LoD reported for HPV-16 L1. It opens the door for further investigation with other electrode platforms and realization of PoC diagnostic devices for screening and testing of HPV biomarkers for cervical cancer.

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A label free nanosensing platform for the detection of cervical cancer through analysis of ultratrace DNA hybridization

Cited by 12 publications

References 28 publications

RETRACTED: Ultra-Sensitive Biosensor with Simultaneous Detection (of Cancer and Diabetes) and Analysis of Deformation Effects on Dielectric Rods in Optical Microstructure

RETRACTED: Ultra-Sensitive Biosensor with Simultaneous Detection (of Cancer and Diabetes) and Analysis of Deformation Effects on Dielectric Rods in Optical Microstructure

An Electroanalytical Flexible Biosensor Based on Reduced Graphene Oxide-DNA Hybrids for the Early Detection of Human Papillomavirus-16

Electrochemical Immunosensor for Ultra-Low Detection of Human Papillomavirus Biomarker for Cervical Cancer

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