A mitochondrial-targetable fluorescent probe based on high-quality InP quantum dots for the imaging of living cells

Zhang, Yanbin; Qiao, Lulu; Zhang, Zhiqiang; Liu, Yongfang; Li, Linsong; Shen, Huaibin; Zhao, Mei‐Xia

doi:10.1016/j.matdes.2022.110736

Cited by 14 publications

(4 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…CuInS 2 QDs are I-III-VI 2 semiconductor nanocrystals and does not contain any toxic heavy metals. They can provide PL emission ranging from the visible to the NIR, and have broad absorption, size-tunable photoluminescence (PL), excellent optical, and chemical stability [ 20 , 21 ]. However, the conventional synthesis of CuInS 2 QDs usually employs toxic organic solvents and the hydrophobic QDs are not appropriate for their application in biomedical fields.…”

Section: Introductionmentioning

confidence: 99%

Green synthesis of glyco-CuInS2 QDs with visible/NIR dual emission for 3D multicellular tumor spheroid and in vivo imaging

et al. 2023

View full text Add to dashboard Cite

Glyco-quantum dots (glyco-QDs) have attracted significant interest in bioimaging applications, notably in cancer imaging, because they effectively combine the glycocluster effect with the exceptional optical properties of QDs. The key challenge now lies in how to eliminate the high heavy metal toxicity originating from traditional toxic Cd-based QDs for in vivo bioimaging. Herein, we report an eco-friendly pathway to prepare nontoxic Cd-free glyco-QDs in water by the “direct” reaction of thiol-ending monosaccharides with metal salts precursors. The formation of glyco-CuInS2 QDs could be explained by a nucleation-growth mechanism following the LaMer model. As-prepared four glyco-CuInS2 QDs were water-soluble, monodispersed, spherical in shape and exhibited size range of 3.0–4.0 nm. They exhibited well-separated dual emission in the visible region (500–590 nm) and near-infrared range (~ 827 nm), which may be attributable to visible excitonic emission and near-infrared surface defect emission. Meanwhile, the cell imaging displayed the reversibly distinct dual-color (green and red) fluorescence in tumor cells (HeLa, A549, MKN-45) and excellent membrane-targeting properties of glyco-CuInS2 QDs based on their good biorecognition ability. Importantly, these QDs succeed in penetrating uniformly into the interior (the necrotic zone) of 3D multicellular tumor spheroids (MCTS) due to their high negative charge (zeta potential values ranging from − 23.9 to − 30.1 mV), which overcame the problem of poor penetration depth of existing QDs in in vitro spheroid models. So, confocal analysis confirmed their excellent ability to penetrate and label tumors. Thus, the successful application in in vivo bioimaging of these glyco-QDs verified that this design strategy is an effective, low cost and simple procedure for developing green nanoparticles as cheap and promising fluorescent bioprobes.

show abstract

Section: Introductionmentioning

confidence: 99%

Green synthesis of glyco-CuInS2 QDs with visible/NIR dual emission for 3D multicellular tumor spheroid and in vivo imaging

et al. 2023

View full text Add to dashboard Cite

show abstract

“…In this approach, the probe identifies the specific target and detaches from the nanomaterials to initiate luminescence. Currently, the most common nanomaterials used for this purpose are gold nanoparticles (AuNPs) [62] , graphene oxide (GO) [29] , carbon nanotubes [91] , quantum dots (QDs) [123] , molybdenum disulfide (MoS2) [124] , etc. Nanomaterials that can function as quenching agents have better probe-binding abilities, light-bleaching resistance, and enzymatic degradation abilities than other quenching agents and result in higher detection sensitivity.…”

Section: Imaging Unitmentioning

confidence: 99%

Recent advances in living cell nucleic acid probes based on nanomaterials for early cancer diagnosis

Liu,

Shi,

et al. 2024

Asian Journal of Pharmaceutical Sciences

View full text Add to dashboard Cite

“…[117][118][119][120] The surface modification of QDs can also be achieved by using a highly branched polyethyleneimine (PEI), an amphiphilic dendrimer, by ligand exchange. [121][122][123] In order to improve the stability and solubility of QDs, these bidentate thiol compounds (dihydrolipoic acid, DHLA) were prepared, which produced highly fluorescent QDs that exhibited great long-term colloidal stability over a wide range of conditions. [124][125][126][127] In recent years, multidentate thiol compounds have been developed as functional ligands that provide better optical properties and stability for QDs, 128 which is one of the development trends of the ligand exchange method for QDs modification.…”

Section: Overview Of Qd-flisamentioning

confidence: 99%

Recent advances in quantum dot-based fluorescence-linked immunosorbent assays

Fan

Zhao

et al. 2023

Nanoscale

View full text Add to dashboard Cite

show abstract

A mitochondrial-targetable fluorescent probe based on high-quality InP quantum dots for the imaging of living cells

Cited by 14 publications

References 47 publications

Green synthesis of glyco-CuInS2 QDs with visible/NIR dual emission for 3D multicellular tumor spheroid and in vivo imaging

Green synthesis of glyco-CuInS2 QDs with visible/NIR dual emission for 3D multicellular tumor spheroid and in vivo imaging

Recent advances in living cell nucleic acid probes based on nanomaterials for early cancer diagnosis

Recent advances in quantum dot-based fluorescence-linked immunosorbent assays

Contact Info

Product

Resources

About