A Novel Process for Rapid Mass Propagation of Santalum Album L. In Liquid Media and Bioreactor

Optimization of Different Factors for Initiation of Somatic Embryogenesis in Suspension Cultures in Sandalwood (Santalum album L.)

“…Besides, the destruction caused by the pandemic spike disease, hemiparasitic nature and deliberate-cultivating character of the plant dictated investigation in the direction of the extension of modern unconventional tools of in vitro propagation, namely callusing, in the early 1960s [10]. Later on, attempts of somatic embryo invention [11] and maturation in bioreactors [12,13] were successfully documented. In addition, the uniform and synchronous suspension cultures make them suitable for the creation of phytochemicals at a large scale.…”

“…In addition, the uniform and synchronous suspension cultures make them suitable for the creation of phytochemicals at a large scale. Substantial work has been done in sandalwood by several scientists throughout the world so far by employing an array of explants for inducing morphogenesis followed by plantlet regeneration with varying level of accomplishment; for instance, embryos [10,14], hypocotyls [14][15][16], shoot tips [17,18], nodal segments [19][20][21][22], leaf discs [23,24], seedlings [25], endosperms [15,16], cotyledons [14] and protoplasts [26] have been used jointly with cell suspension cultures [11][12][13]27].…”

Culture of East Indian sandalwood tree somatic embryos in air-lift bioreactors for production of santalols, phenolics and arabinogalactan proteins

“…To this end, a bioreactor-based production system using a liquid medium was developed ( Das et al 1998 ). However, the scale-up study reported earlier ( Das et al 1999 ) did not address the detailed investigation of biochemicals. In this study, we compare the culture of SEs in 2- and 10-L air-lift bioreactors as a biotechnological means of propagation for this endangered species in terms of the growth and biochemical characteristics.…”

“…callusing ( Rangaswamy and Rao 1963 ). Further efforts on somatic embryo (SE) production ( Bapat et al 1990 ) and maturation in air-lift bioreactors ( Das et al 1998 , 1999 ) have also been successful.…”