A red-emission probe for intracellular biothiols imaging with a large Stokes shift

A lysosome-targeted fluorescent probe based on a BODIPY structure for Cys/Hcy detection

“…The pH experiment was conducted by using a pH/ATC electrode (Sartorius). The new compounds were analysed by 1 H-NMR and 13 C-NMR spectroscopy using a 400 MHz or 500 MHz spectrometer (Bruker). The synthesis processes of the NBD-B-T probe are given in the ESI.…”

“…[4][5][6][7][8][9][10][11][12] Hcy is a non-protein amino acid that can be broken down into Cys by a sulfurization reaction. [13][14][15] Abnormal accumulation of Hcy in the body can lead to various health threatening diseases, such as Alzheimer's disease, Parkinson's disease and cancer. 16 Therefore, it is particularly important to explore a sensitive, fast and highly selective method to monitor Cys and Hcy in biological systems.…”

“…conrmed by1 H-NMR,13 C-NMR, high-resolution mass spectrometry (HRMS) (Fig.S1-S12, ESI †).Spectral response of NBD-B-T towards Cys/HcyThe optical properties of NBD-B-T and B-T-OH were also investigated. The absorption and uorescence spectra of the NBD-B-T probe and its response for Cys and Hcy were measured in PBS/DMF buffer (v/v = 1/1, pH = 7.4).…”

Structure modulation on fluorescent probes for biothiols and the reversible imaging of glutathione in living cells

“…Fluorescent probes have attracted considerable attentions in the determination of the variety and content of bio-species, ascribed to their advantages of high sensitivity, facile operation, visualization and noninvasiveness. [15][16][17][18][19][20][21][22] A variety of uorophores have been utilized to construct uorescent probes for biothiol detection, which covered a wide range of emission signals from green to near-infrared uorescence that could satisfy the requirements in different situations. [23][24][25][26][27][28][29] And some probes could discriminate Cys, Hcy and GSH selectively.…”

A cysteine-selective fluorescent probe for monitoring stress response cysteine fluctuations

“…And numerous fluorescent probes have been widely used in the imaging of biologically reactive species to reveal life phenomena in cells or in vivo in recent years, [6][7][8][9][10][11] including related research on cysteine monitoring. A series of receptors were selected and used as recognition groups for probe design, such as 2,4-dinitrobenzenesulfonyl, [12][13][14][15][16] acryloyl, [17][18][19][20][21][22][23] a,b-unsaturated ketone, 24 nitrobenzodioxazole (NBD) and others, [25][26][27] and we have summarized and compared the differences among them (Fig. 1A and Table S1, ESI †).…”