A reliable high-performance liquid chromatography with ultraviolet detection for the determination of sulfonamides in honey

Granja, Rodrigo H M M; Niño, Alfredo M. Montes; Rabone, F; Salerno, Alessandro G.

doi:10.1016/j.aca.2008.02.048

Cited by 36 publications

(16 citation statements)

References 1 publication

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“…The extraction efficiencies of analytes by DLLME-HPLC-DAD were compared with other reported methods such as SPE-HPLC-UV 8,22 and SPE-HPLC-MS 23 from the viewpoint of the extraction time, relative standard deviation (RSD), limits of detection (LOD) and linear range (LR). As listed in Table-3, DLLME showed lower RSD (0.172-1.549 %) and LODs (0.8-1.7 µg L -1 ) and much wider linear range, (0.01-10.0 µg mL -1 ) in comparison with SPE-HPLC.…”

Section: Comparison Of Dllme With Spementioning

confidence: 99%

Simultaneous Determination of Sulfonamides in Honey by Dispersive Liquid-liquid Microextraction Combined with High-Performance Liquid Chromatography

Chi¹,

Zhang²,

Dong³

et al. 2014

Asian J. Chem.

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Sulfonamides are extensively used in veterinary practice for the treatment of various bacterial infections. Use of sulfonamides in food producing animals may result in sulfonamide residues being present in the edible products. The European Community has established maximum residue limits for sulfonamides in foods of animal origin at 0.1 ppm to ensure the safety of food to the consumers 1. Honey bee larvae are susceptible to American foulbrood or European foulbrood, disease caused by the organism Bacillus larvae, which can devastate hives. Sulfonamides are relatively stable chemotherapeutics known to control this disease 2 but they are not permitted to use for this purpose in most countries. Nevertheless, due to their low price and steady antibiosis effectiveness, illegal use of sulfonamides still exists. So, it is very important to develop a sensitive, rapid and simple method for the determination of sulfonamides. Up to now, a variety of analytical methods have been used to measure sulfonamide residues in biological materials. The main approaches for identification and quantification of sulfonamide residues include: enzyme immunoassay 3-5 , thin-layer chromatography 6,7 and liquid chromatography 8-10. Aiming of cleaning up and concentrating the analytes of interest and

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Section: Comparison Of Dllme With Spementioning

confidence: 99%

Simultaneous Determination of Sulfonamides in Honey by Dispersive Liquid-liquid Microextraction Combined with High-Performance Liquid Chromatography

Chi¹,

Zhang²,

Dong³

et al. 2014

Asian J. Chem.

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“…Several methods using CE with UV , fluorescence , electrochemical (ED) , and MS detection have been used for the determination of these drugs in meat, tissues, tablets, and human urine samples, respectively. But the most commonly used methods include LC using UV diode array , fluorescence , MS , or MS/MS detection . These methods require significant amounts of time associated with clean‐up steps, sophisticated instrumentation, and skilled operators.…”

Section: Introductionmentioning

confidence: 99%

Determination of sulfonamides in milk samples by HPLC with amperometric detection using a glassy carbon electrode modified with multiwalled carbon nanotubes

Bueno

Contento

Ríos

2014

J of Separation Science

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A sensitive and accurate method for determining five sulfonamides based on HPLC with amperometric detection and using a glassy carbon electrode modified with multiwalled carbon nanotubes is proposed. Optimal conditions for the quantitative separation of selected sulfonamides were studied, and glassy carbon electrodes with and without modification with carbon nanotubes were systematically investigated as electrodic materials. Statistical analysis of the obtained results demonstrated that these modified electrodes achieved considerably better stability and sensitivity than the conventional unmodified ones. Detection limits were in the 1.2-6.0 ng/mL range. The usefulness of the method was demonstrated by the analysis of milk samples, taking into account the European legislation on residues in food products, following both a screening method to classify the samples and a confirmation method to provide more detailed information in the case of positive samples.

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“…Several conventional methods have been successfully applied for the separation and determination of the sulfonamide content in different samples including gas chromatography (GC), gas chromatography-mass spectrometry (GC/ MS), capillary electrophoresis (CE) [6,7], enzyme-linked immunosorbent assay (ELISA) [8,9], thin-layer chromatography (TLC), high-performance liquid chromatography (HPLC-UV) [10][11][12][13][14][15][16][17], and high-performance liquid chromatography-mass spectrometry (HPLC/MS) [18][19][20][21][22][23][24][25]. Although the mentioned methods offer good resolution of organic compound, their sensitivities are often in sufficient for direct determination of trace sulfonamides residue in food.…”

Section: Introductionmentioning

confidence: 99%

“…To increase the performance and sensitivity in HPLC-EC by means of fully automation, in this work, we used online Oasis HLB SPE procedure to extend our previous efforts on the determination of sulfonamides instead of using offline SPE before their HPLC separation. The common detectors for chromatography with online SPE are ultraviolet (UV) [10][11][12][13][14][15][16][17], fluorescence [1,4,37] and MS [18][19][20][21][22][23][24][25]. Even though these methods provide high sensitivity and selectivity, there are the expensive equipment and a requirement for significant labor and analytical resources.…”

Section: Introductionmentioning

confidence: 99%

Automated Online Solid-Phase Extraction Coupled with Sequential Injection-HPLC-EC System for the Determination of Sulfonamides in Shrimp

Chantarateepra

Siangproh

Motomizu

et al. 2012

International Journal of Electrochemistry

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The use of fully automated online solid-phase extraction (SPE) coupled with sequential injection analysis, high-performance liquid chromatography (HPLC), and electrochemical detection (EC) for the separation and determination of sulfonamides has been developed. A homemade microcolumn SPE system coupled with sequential injection analysis (SIA) was used to automate the sample cleanup and extraction of sulfonamides. The optimal flow rate of sample loading and elution was found to be 10 μL/s, and optimal elution time of zone was 20–24 s. Under the optimal conditions, a linear relationship between peak area and sulfonamide concentrations was obtained in the range of 0.01–8.0 μg mL−1. Detection limits for seven sulfonamides were between 1.2 ng mL−1and 11.2 ng mL−1. The proposed method has been applied for the determination of sulfonamides in shrimp. Recoveries in the range of 84–107% and relative standard deviations (RSDs) below 6.5% for intraday and 13% for inter-day were received for three concentration levels of spiking. The results showed that the present method was simple, rapid, accurate and highly sensitive for the determination of sulfonamides.

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A reliable high-performance liquid chromatography with ultraviolet detection for the determination of sulfonamides in honey

Cited by 36 publications

References 1 publication

Simultaneous Determination of Sulfonamides in Honey by Dispersive Liquid-liquid Microextraction Combined with High-Performance Liquid Chromatography

Simultaneous Determination of Sulfonamides in Honey by Dispersive Liquid-liquid Microextraction Combined with High-Performance Liquid Chromatography

Determination of sulfonamides in milk samples by HPLC with amperometric detection using a glassy carbon electrode modified with multiwalled carbon nanotubes

Automated Online Solid-Phase Extraction Coupled with Sequential Injection-HPLC-EC System for the Determination of Sulfonamides in Shrimp

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