A simplified method for detecting pathogenic Yersinia enterocolitica in slaughtered pig tonsils

Fondrevez, M.; Labbé, A.; Houard, Emmanuelle; Fravalo, Philippe; Madec, F.; Denis, Martine

doi:10.1016/j.mimet.2010.09.012

Cited by 25 publications

(28 citation statements)

References 30 publications

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“…Isolation of pathogenic Y. enterocolitica from naturally contaminated samples without enrichment is seldom successful (Fredriksson-Ahomaa and Korkeala, 2003); however, for tonsil samples, direct plating is a fast alternative as in most samples background flora on a selective agar medium is minimal and the number of pathogenic Y. enterocolitica is sufficiently high (Fredriksson-Ahomaa et al, 2007;Van Damme et al, 2010). A disadvantage of current conventional media is the difficulty to distinguish pathogenic Y. enterocolitica from non-pathogenic yersiniae and many other Gram-negative bacteria (de Boer, 1992;Fondrevez et al, 2010). To facilitate this differentiation, Weagant (2008) recently designed a chromogenic agar medium, called Y. enterocolitica chromogenic medium (YeCM).…”

Section: Introductionmentioning

confidence: 99%

“…When monitoring Y. enterocolitica in pigs at slaughter, tonsils are regarded as the sample of choice (Fredriksson-Ahomaa et al, 2001;Nesbakken et al, 2003). Swab samples from tonsils have been used in several studies (Andersen et al, 1991;Fondrevez et al, 2010;Kechagia et al, 2007), since sampling and processing is easier and less time-consuming compared to tissue samples. Therefore, this study evaluates if swab samples have a good predictive value for the presence of pathogenic Y. enterocolitica in pig tonsils.…”

Section: Introductionmentioning

confidence: 99%

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Effect of Sampling and Short Isolation Methodologies on the Recovery of Human PathogenicYersinia enterocoliticafrom Pig Tonsils

Damme

Berkvens

Zutter

2012

Foodborne Pathogens and Disease

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effect of Sampling and Short Isolation Methodologies on the Recovery of Human PathogenicYersinia enterocoliticafrom Pig Tonsils

Damme

Berkvens

Zutter

2012

Foodborne Pathogens and Disease

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“…Nevertheless, the combination usage of modified CIN with a chromogenic media, such as CHROMagar Yersinia [15] and YECA [18], and YeCM [19], may help eliminate non-pathogenic Yersinia without the need to conduct additional biochemical tests [23]. This combination usage may also reduce the false-positive results caused by wrong colour interpretations by the user [22].…”

Section: Discussionmentioning

confidence: 99%

“…At the same time, the true positive Y. enterocolitica isolates increased by nearly 27% and 10% on modified CIN compared to CIN in artificially prepared bacterial mixture and artificially contaminated pork, respectively (Table 4). The capability of modified CIN in reducing the number of false positive Y. enterocolitica isolates, especially in eliminating H 2 S-producing Citrobacter spp., M. morganii, P. rettgeri, A. hydrophila, E. cloacae can shorten the processing time and reduce the workload and costs associated with biochemical assays that were reported in many studies [13], [14], [15], [16], [23], [32]. Higher recovery rates from the modified CIN compared with that of CIN was further demonstrated using naturally contaminated samples (rectal swabs from swine, Table 2), indicating the modified CIN improved the recovery of Y. enterocolitica even on actual samples.…”

Section: Discussionmentioning

confidence: 99%

“…The purple, light denim blue, and light pink colours are frequently miss-interpreted as mauve and cannot be ruled out as possible presumptive colonies [22]. Furthermore, even if the chromogenic media are useful for differentiating the virulent from the non-virulent biotypes, the use of CIN is still required for the isolation step prior to plating on chromogenic media [23].…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of a Modified Cefsulodin-Irgasan-Novobiocin Agar for Isolation of Yersinia spp

et al. 2014

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Y. enterocolitica and Y. pseudotuberculosis are important food borne pathogens. However, the presence of competitive microbiota makes the isolation of Y. enterocolitica and Y. pseudotuberculosis from naturally contaminated foods difficult. We attempted to evaluate the performance of a modified Cefsulodin-Irgasan-Novobiocin (CIN) agar in the differentiation of Y. enterocolitica from non-Yersinia species, particularly the natural intestinal microbiota. The modified CIN enabled the growth of Y. enterocolitica colonies with the same efficiency as CIN and Luria-Bertani agar. The detection limits of the modified CIN for Y. enterocolitica in culture medium (10 cfu/ml) and in artificially contaminated pork (104 cfu/ml) were also comparable to those of CIN. However, the modified CIN provided a better discrimination of Yersinia colonies from other bacteria exhibiting Yersinia-like colonies on CIN (H2S-producing Citrobacter freundii, C. braakii, Enterobacter cloacae, Aeromonas hydrophila, Providencia rettgeri, and Morganella morganii). The modified CIN exhibited a higher recovery rate of Y. enterocolitica from artificially prepared bacterial cultures and naturally contaminated samples compared with CIN. Our results thus demonstrated that the use of modified CIN may be a valuable means to increase the recovery rate of food borne Yersinia from natural samples, which are usually contaminated by multiple types of bacteria.

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Diversity of Yersinia enterocolitica isolated from pigs in a French slaughterhouse over 2 years

et al. 2018

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The pig is one of the main reservoirs of Yersinia enterocolitica strains pathogenic to humans. A description of the Y. enterocolitica population in this reservoir, and accurate discriminatory techniques for typing isolates are needed for prevention, outbreak investigation, and surveillance. This study investigates the genetic diversity of pathogenic Y. enterocolitica isolates obtained from pig tonsils in a French pig slaughterhouse in 2009 (S1) and 2010 (S2). The use of Pulsed‐Field Gel Electrophoresis (PFGE) and MLVA as typing techniques was also compared and evaluated. First, a total of 167 isolates (12 of biotype 3 recovered during S1, and 155 of biotype 4 recovered during S1 and S2) were typed by PFGE using the XbaI enzyme. MLVA was then tested on all the biotype 3 isolates in addition to 70 selected biotype 4 isolates recovered over the 2 years. PFGE generated two specific XbaI‐PFGE profiles for biotype 3 isolates. Nine XbaI profiles were obtained for biotype 4, with a higher diversity (ID = 0.599) than biotype 3 (ID = 0.167). Two out of the nine XbaI profiles were reported during both surveys and at different months. MLVA improved the differentiation between isolates; the index of diversity reached 0.621 and 0.958, respectively, for biotype 3 (three MLVA types) and biotype 4 (32 MLVA types). The MLVA types for biotype 4 differed over the two surveys, but some isolates with different MLVA types were genetically closely related. This study provides an initial evaluation of the genetic diversity of Y. enterocolitica strains isolated from pigs in France. We show that some PFGE profiles are maintained in the pig production sector, and, through MLVA, that part of the Y. enterocolitica population remained genetically close over the two years. MLVA proved its effectiveness as a tool for investigating pathogenic Y. enterocolitica strains isolated from pigs.

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A simplified method for detecting pathogenic Yersinia enterocolitica in slaughtered pig tonsils

Cited by 25 publications

References 30 publications

Effect of Sampling and Short Isolation Methodologies on the Recovery of Human PathogenicYersinia enterocoliticafrom Pig Tonsils

Effect of Sampling and Short Isolation Methodologies on the Recovery of Human PathogenicYersinia enterocoliticafrom Pig Tonsils

Evaluation of a Modified Cefsulodin-Irgasan-Novobiocin Agar for Isolation of Yersinia spp

Diversity of Yersinia enterocolitica isolated from pigs in a French slaughterhouse over 2 years

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