Accelerated Photo-Induced Hydrophilicity Promotes Osseointegration: An Animal Study

Jimbo, Ryo; Ono, Daisuke; Hirakawa, Yuko; Odatsu, Tetsurou; Tanaka, Tsunehiro; Saeki, Takashi

doi:10.1111/j.1708-8208.2009.00179.x

Cited by 48 publications

(49 citation statements)

References 23 publications

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“…It is well known that plasma fibronectin binds more to hydrophilic surfaces [36], whereas albumin binds more to hydrophobic surfaces [37]. Anodic oxidized Ti surfaces have been reported to present hydrophilicity [38,39]. It has also been reported that anodic oxidized Ti surfaces have high surface energy [40,41], which is essential for maintaining surface hydrophilicity [42].…”

Section: Discussionmentioning

confidence: 99%

Protein Adsorption to Surface Chemistry and Crystal Structure Modification of Titanium Surfaces

Jimbo¹,

Ivarsson²,

Koskela³

et al. 2010

JOMR

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ObjectivesTo observe the early adsorption of extracellular matrix and blood plasma proteins to magnesium-incorporated titanium oxide surfaces, which has shown superior bone response in animal models.Material and MethodsCommercially pure titanium discs were blasted with titanium dioxide (TiO2) particles (control), and for the test group, TiO2 blasted discs were further processed with a micro-arc oxidation method (test). Surface morphology was investigated by scanning electron microscopy, surface topography by optic interferometry, characterization by X-ray photoelectron spectroscopy (XPS), and by X-ray diffraction (XRD) analysis. The adsorption of 3 different proteins (fibronectin, albumin, and collagen type I) was investigated by an immunoblotting technique.ResultsThe test surface showed a porous structure, whereas the control surface showed a typical TiO2 blasted structure. XPS data revealed magnesium-incorporation to the anodic oxide film of the surface. There was no difference in surface roughness between the control and test surfaces. For the protein adsorption test, the amount of albumin was significantly higher on the control surface whereas the amount of fibronectin was significantly higher on the test surface. Although there was no significant difference, the test surface had a tendency to adsorb more collagen type I.ConclusionsThe magnesium-incorporated anodized surface showed significantly higher fibronectin adsorption and lower albumin adsorption than the blasted surface. These results may be one of the reasons for the excellent bone response previously observed in animal studies.

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Section: Discussionmentioning

confidence: 99%

Protein Adsorption to Surface Chemistry and Crystal Structure Modification of Titanium Surfaces

Jimbo¹,

Ivarsson²,

Koskela³

et al. 2010

JOMR

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“…On photocatalytic TiO 2 surfaces, UV light generates free radicals available to mineralize adhered contaminants (such as bacteria), but also creates a superhydrophilic surface due to increased hydroxylation [47]. This makes for an efficient, on-demand self cleaning surface, but it has also been shown that pretreatments with UV light can enhance protein adsorption, cell attachment and osteoconductivity on Ti implants, largely due to removal of adhered hydrocarbons [48,49]. Moreover, Ag-doped TiO 2 has displayed higher photocatalytic activity and stronger antibacterial effect than regular TiO 2 , due to improved charge separation, making it an interesting option as a multifunctional biomedical coating [50].…”

Section: Discussionmentioning

confidence: 99%

Reactive combinatorial synthesis and characterization of a gradient Ag–Ti oxide thin film with antibacterial properties

et al. 2015

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Growing demand for orthopedic and dental implants has spurred researchers to develop multifunctional coatings, combining tissue integration with antibacterial features. A possible strategy to endow titanium (Ti) with antibacterial properties is by incorporating silver (Ag), but designing a structure with adequate Ag + release while maintaining biocompatibility has been shown difficult. To further explore the composition-structure-property relationships between Ag and Ti, and its effects against bacteria, this study utilized a combinatorial approach to manufacture and test a single sample containing a binary Ag-Ti oxide gradient. The sample, sputter deposited in a reactive (O 2 ) environment using a custom built combinatorial physical vapor deposition (PVD) system, was shown to be effective against Staphylococcus aureus with viability reductions ranging from 17 to above 99 %, depending on amount of Ag + released from its different parts. The Ag content along the gradient ranged from 35 to 62 wt%, but it was found that structural properties such as varied porosity and degree of crystallinity, rather than amount of incorporated Ag governed the Ag + release and resulting antibacterial activity. The coating also demonstrated in vitro apatite forming abilities, where structural variety along the sample was shown to alter the hydrophilic behavior, with degree of hydroxyapatite (HA) deposition varying accordingly. By means of combinatorial synthesis, a single gradient sample was able to display intricate compositional and structural features affecting its biological response, which would otherwise require a series of coatings. The current findings suggest that future implant coatings incorporating Ag as an antibacterial agent could be structurally enhanced to better suit clinical requirements.

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“…Irrespective of the biomaterial, surface topography, or wettability status of the surface, the initial contact with blood will rapidly attract proteins [1], which in turn will initiate the process of bone formation [2, 3]. In fact, protein adsorption to the implant surface has been suggested to be important for the osteoconduction stage of osseointegration [4–6].…”

Section: Introductionmentioning

confidence: 99%

The Effect of Laminin-1-Doped Nanoroughened Implant Surfaces: Gene Expression and Morphological Evaluation

Schwartz-Filho

Bougas

Coelho

et al. 2012

International Journal of Biomaterials

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Aim. This study aimed to observe the morphological and molecular effect of laminin-1 doping to nanostructured implant surfaces in a rabbit model. Materials and Methods. Nanostructured implants were coated with laminin-1 (test; dilution, 100 μg/mL) and inserted into the rabbit tibiae. Noncoated implants were used as controls. After 2 weeks of healing, the implants were removed and subjected to morphological analysis using scanning electron microscopy (SEM) and gene expression analysis using the real-time reverse transcriptase-polymerase chain reaction (RT-PCR). Results. SEM revealed bony tissue attachment for both control and test implants. Real-time RT-PCR analysis showed that the expression of osteoblast markers RUNX-2, osteocalcin, alkaline phosphatase, and collagen I was higher (1.62-fold, 1.53-fold, 1.97-fold, and 1.04-fold, resp.) for the implants modified by laminin-1 relative to the control. All osteoclast markers investigated in the study presented higher expression on the test implants than controls as follows: tartrate-resistant acid phosphatase (1.67-fold), calcitonin receptor (1.35-fold), and ATPase (1.25-fold). The test implants demonstrated higher expression of inflammatory markers interleukin-10 (1.53-fold) and tumour necrosis factor-α (1.61-fold) relative to controls. Conclusion. The protein-doped surface showed higher gene expression of typical genes involved in the osseointegration cascade than the control surface.

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Accelerated Photo-Induced Hydrophilicity Promotes Osseointegration: An Animal Study

Abstract: The results proved that the enhanced photo-induced hydrophilicity of the NH(4) F-HF(2) -modified anodized implants promoted bone apposition during the early stages of osseointegration.

Cited by 48 publications

References 23 publications

Protein Adsorption to Surface Chemistry and Crystal Structure Modification of Titanium Surfaces

Protein Adsorption to Surface Chemistry and Crystal Structure Modification of Titanium Surfaces

Reactive combinatorial synthesis and characterization of a gradient Ag–Ti oxide thin film with antibacterial properties

The Effect of Laminin-1-Doped Nanoroughened Implant Surfaces: Gene Expression and Morphological Evaluation

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